A predominant β-CGTase G1 engineered to elucidate the relationship between protein structure and product specificity

• Main Authors: Goh, Kin Mau, Mahadi, Nor Muhammad, Hassan, Osman, Rahman, Raja Noor Zaliha Raja Abdul, Illias, Rosli Md
• Format: Article
• Published: Elsevier B.V. 2009
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/7268/; http://dx.doi.org/10.1016/j.molcatb.2008.09.016
• Institution: Universiti Teknologi Malaysia

Low reaction yields and the high cost of obtaining a single type of pure CD make γ-CD costly. Using rational design and with the aid of 3D modeling structures, recombinant CGTase from Bacillus sp. G1 was molecularly engineered with the aim of producing a higher percentage of γ-CD. A single mutatio...