Bioprocess strategies to optimize thermostable phytase production by Escherichia coli B121 (DE3) when induced with lactose

• Main Authors: Othman, N. Z., Ramli, S., Malek, R. A., Sarmidi, M. R., Hatti-Kaul, R., El Enshasy, H. A.
• Format: Conference or Workshop Item
• Published: AIChE 2016
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/73691/; https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019033038&partnerID=40&md5=d8f6889f3f395a0f6e4658e175c4e238
• Institution: Universiti Teknologi Malaysia

The recombinant Escherichia coli BL21(DE3) that carries a thermostable phytase gene from Bacillussp. MD2 and lactose as an inducer was used in this study. This research aims to study the influenced of induction strategy to maximize expression of phytase by E. coli BL21(DE3) and leakage of phytase ou...