Recovery of rutin from labisia pumila extract using solid phase extraction

• Main Authors: Chua, L. S., Ruzlan, N. N., Sarmidi, M. R.
• Format: Article
• Language: English
• Published: Slovensko Kemijsko Drustvo 2017
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/75135/1/MohamadRojiSarmidi2017_RecoveryofRutinfromLabisiaPumilaExtract.pdf; http://eprints.utm.my/id/eprint/75135/; https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040349994&doi=10.17344%2facsi.2017.3500&partnerID=40&md5=13ac42a4a46ceb0100e45f9cd18993db
• Institution: Universiti Teknologi Malaysia

Reflux extraction was used to prepare crude extract from the leaves of Labisia pumila var. Alata using 60% methanol. The crude extract was subsequently fractionated by C18 solid phase extraction to recover high yield of rutin using 20-100% methanol. The volume of eluent to recover rutin was found to decrease with the increase of methanol concentration. The recovery of rutin was increased from 20 to 80% methanol system, but slightly decreased in the 100% methanol system. Approximately, 70% of rutin could be recovered using the 80% methanol system. This solvent system also appears to have the lowest distance (9.44 MPa1/2) for rutin as estimated by Hansen solubility. The recovered rutin rich fraction could achieve up to 3.96 mg/g of fraction which was about 4-fold increment from the crude extract. The increment was also noticed for its antioxidant capacity expressed as scavenging activity which was 2 times higher than crude extract. A portion of water (20%) in the 80% methanol system is important to improve the yield of rutin. Rutin is a glycosylated flavonol, and therefore a small portion of water could enhance its elution compared to the lower performance of 100% methanol in rutin recovery.