Optimization of biohydrogen production by Enterococcus SP. NF6 using starch as substrate
The rising gap between the demand for fossil fuel-based energy and insufficient energy supply has led to the vast investigation of potential alternative clean energy, biohydrogen. In this study, starch utilizing bacteria Enterococcus sp. NF6 was isolated from cow manure; the effective starch utiliza...
Saved in:
Main Author: | |
---|---|
Format: | Thesis |
Language: | English |
Published: |
2017
|
Subjects: | |
Online Access: | http://eprints.utm.my/id/eprint/78560/1/DharsigahNBaniearSalvamMFBME2017.pdf http://eprints.utm.my/id/eprint/78560/ http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:108143 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Universiti Teknologi Malaysia |
Language: | English |
id |
my.utm.78560 |
---|---|
record_format |
eprints |
spelling |
my.utm.785602018-08-29T07:31:51Z http://eprints.utm.my/id/eprint/78560/ Optimization of biohydrogen production by Enterococcus SP. NF6 using starch as substrate N. Baniear Salvam, Dharsigah TP Chemical technology The rising gap between the demand for fossil fuel-based energy and insufficient energy supply has led to the vast investigation of potential alternative clean energy, biohydrogen. In this study, starch utilizing bacteria Enterococcus sp. NF6 was isolated from cow manure; the effective starch utilization and biohydrogen production of the biocatalyst were investigated. To investigate the optimum conditions for biohydrogen production, batch fermentations were conducted by optimization of physicochemical parameters using one factor at a time (OFAT) method using Modified Reinforced Clostridial Media (MRCM) as fermentation media. Batch fermentations were carried out at several temperatures (30°C, 37°C & 45°C), initial medium pH (5.5, 7.0, 8.0 & 9.0) and initial substrate concentration of (0.5 g/L, 1 g/L & 3g/L). The highest biohydrogen production obtained at pH 5.5, incubation temperature of 37°C, and initial substrate concentration of 1g/L. These conditions, the biohydrogen production rate is 0.23 mL/H, with 100% of substrate utilization, 0.0790 h-1 of specific growth rate and maximum biohydrogen production of 1.85 mL. The outcome suggests the potential use of Enterococcus sp. NF6 for biohydrogen production in a single culture system. 2017-07 Thesis NonPeerReviewed application/pdf en http://eprints.utm.my/id/eprint/78560/1/DharsigahNBaniearSalvamMFBME2017.pdf N. Baniear Salvam, Dharsigah (2017) Optimization of biohydrogen production by Enterococcus SP. NF6 using starch as substrate. Masters thesis, Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering. http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:108143 |
institution |
Universiti Teknologi Malaysia |
building |
UTM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Teknologi Malaysia |
content_source |
UTM Institutional Repository |
url_provider |
http://eprints.utm.my/ |
language |
English |
topic |
TP Chemical technology |
spellingShingle |
TP Chemical technology N. Baniear Salvam, Dharsigah Optimization of biohydrogen production by Enterococcus SP. NF6 using starch as substrate |
description |
The rising gap between the demand for fossil fuel-based energy and insufficient energy supply has led to the vast investigation of potential alternative clean energy, biohydrogen. In this study, starch utilizing bacteria Enterococcus sp. NF6 was isolated from cow manure; the effective starch utilization and biohydrogen production of the biocatalyst were investigated. To investigate the optimum conditions for biohydrogen production, batch fermentations were conducted by optimization of physicochemical parameters using one factor at a time (OFAT) method using Modified Reinforced Clostridial Media (MRCM) as fermentation media. Batch fermentations were carried out at several temperatures (30°C, 37°C & 45°C), initial medium pH (5.5, 7.0, 8.0 & 9.0) and initial substrate concentration of (0.5 g/L, 1 g/L & 3g/L). The highest biohydrogen production obtained at pH 5.5, incubation temperature of 37°C, and initial substrate concentration of 1g/L. These conditions, the biohydrogen production rate is 0.23 mL/H, with 100% of substrate utilization, 0.0790 h-1 of specific growth rate and maximum biohydrogen production of 1.85 mL. The outcome suggests the potential use of Enterococcus sp. NF6 for biohydrogen production in a single culture system. |
format |
Thesis |
author |
N. Baniear Salvam, Dharsigah |
author_facet |
N. Baniear Salvam, Dharsigah |
author_sort |
N. Baniear Salvam, Dharsigah |
title |
Optimization of biohydrogen production by Enterococcus SP. NF6 using starch as substrate |
title_short |
Optimization of biohydrogen production by Enterococcus SP. NF6 using starch as substrate |
title_full |
Optimization of biohydrogen production by Enterococcus SP. NF6 using starch as substrate |
title_fullStr |
Optimization of biohydrogen production by Enterococcus SP. NF6 using starch as substrate |
title_full_unstemmed |
Optimization of biohydrogen production by Enterococcus SP. NF6 using starch as substrate |
title_sort |
optimization of biohydrogen production by enterococcus sp. nf6 using starch as substrate |
publishDate |
2017 |
url |
http://eprints.utm.my/id/eprint/78560/1/DharsigahNBaniearSalvamMFBME2017.pdf http://eprints.utm.my/id/eprint/78560/ http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:108143 |
_version_ |
1643657936104325120 |