Development of an Amperometric Glucose Biosensor Based on the Immobilization of Glucose Oxidase on the Se-MCM-41 Mesoporous Composite

• Main Authors: Yusan, S., Rahman, M. M., Mohamad, N., Arrif, T. M., Latif, A. Z. A., Mohd Aznan, M. A., Wan Nik, W. S. B.
• Format: Article
• Language: English
• Published: Hindawi Limited 2018
• Subjects: QD Chemistry
• Online Access: http://eprints.utm.my/id/eprint/79920/1/SabriyeYusan2018_DevelopmentofanAmperometricGlucoseBiosensor.pdf; http://eprints.utm.my/id/eprint/79920/; http://dx.doi.org/10.1155/2018/2687341
• Institution: Universiti Teknologi Malaysia

A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employed to probe the catalytic behavior of the biosensor. A linear calibration plot is obtained over a wide concentration range of glucose from 1 × 10-5 to 2 × 10-3 M. Under optimal conditions, the biosensor exhibits high sensitivity (0.34 μA·mM-1), low detection limit (1 × 10-4 M), high affinity to glucose (Km = 0.02 mM), and also good reproducibility (R.S.D. 2.8%, n=10) and a stability of about ten days when stored dry at +4°C. Besides, the effects of pH value, scan rate, mediator effects on the glucose current, and electroactive interference of the biosensor were also discussed. As a result, the biosensor exhibited an excellent electrocatalytic response to glucose as well as unique stability and reproducibility.