Site-saturation mutagenesis of mutant L-asparaginase II signal peptide hydrophobic region for improved excretion of cyclodextrin glucanotransferase

• Main Authors: Ismail, A., Illias, R. M.
• Format: Article
• Published: Springer-Verlag 2017
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/81138/; http://dx.doi.org/10.1007/s10295-017-1980-6
• Institution: Universiti Teknologi Malaysia

The excretion of cyclodextrin glucanotransferase (CGTase) into the culture medium offers significant advantages over cytoplasmic expression. However, the limitation of Escherichia coli is its inability to excrete high amount of CGTase outside the cells. In this study, modification of the hydrophobic...