Fractionation and hydrolysis of cellooligosaccharides by Trichoderma reesei Cellobiohydrolase 7A

Energy Security and Chemical Engineering Congress 2019, ESChE 2019; Kuala Lumpur; Malaysia; 17 July 2019 through 19 July 2019; Code 158114 This study aimed to investigate the fractionation and hydrolysis of cellooligosaccharides by Trichoderma reesei Cellobiohydrolase 7A (TrCel7A). The enzyme TrCel...

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Main Authors: Syed Abdul Rahman, Sharifah Annirah, Abang Zaidel, Dayang Norulfairuz
Format: Article
Published: Institute of Physics Publishing 2020
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Online Access:http://eprints.utm.my/id/eprint/87226/
http://dx.doi.org/10.1088/1757-899X/736/4/042022
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Institution: Universiti Teknologi Malaysia
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spelling my.utm.872262020-10-31T12:26:54Z http://eprints.utm.my/id/eprint/87226/ Fractionation and hydrolysis of cellooligosaccharides by Trichoderma reesei Cellobiohydrolase 7A Syed Abdul Rahman, Sharifah Annirah Abang Zaidel, Dayang Norulfairuz TP Chemical technology Energy Security and Chemical Engineering Congress 2019, ESChE 2019; Kuala Lumpur; Malaysia; 17 July 2019 through 19 July 2019; Code 158114 This study aimed to investigate the fractionation and hydrolysis of cellooligosaccharides by Trichoderma reesei Cellobiohydrolase 7A (TrCel7A). The enzyme TrCel7A was purified from enzyme mixture (Celluclast®). Fractionation of soluble cellooligosaccharides was performed using size exclusion chromatography (SEC) and hydrolysis of the cellooligosaccharides with degree of polymerisation (DP) from 3 to 6 was conducted by TrCel7A at 25 °C for 1 h, and the product concentration was analysed by highperformance liquid chromatography (HPLC). HPLC results showed that glucose was produced for each DP of cellooligosaccharides. However, the highest product that achieved for all DP of cellooligosaccharides was cellobiose, followed by cellotriose (in the case of DP 5 and 6) and glucose. The reaction of cellooligosaccharides with DP 3 to 6 during hydrolysis by TrCel7A were modelled to show exactly the cleavage sites for each DP of cellooligosaccharides. The model showed that all cellooligosaccharides was hydrolysed at the first (glucose) and second (cellobiose) glycosidic linkages by TrCel7A. Institute of Physics Publishing 2020 Article PeerReviewed Syed Abdul Rahman, Sharifah Annirah and Abang Zaidel, Dayang Norulfairuz (2020) Fractionation and hydrolysis of cellooligosaccharides by Trichoderma reesei Cellobiohydrolase 7A. IOP Conference Series: Materials Science and Engineering, 736 (4). 042022-042022. ISSN 1757-8981 http://dx.doi.org/10.1088/1757-899X/736/4/042022
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Syed Abdul Rahman, Sharifah Annirah
Abang Zaidel, Dayang Norulfairuz
Fractionation and hydrolysis of cellooligosaccharides by Trichoderma reesei Cellobiohydrolase 7A
description Energy Security and Chemical Engineering Congress 2019, ESChE 2019; Kuala Lumpur; Malaysia; 17 July 2019 through 19 July 2019; Code 158114 This study aimed to investigate the fractionation and hydrolysis of cellooligosaccharides by Trichoderma reesei Cellobiohydrolase 7A (TrCel7A). The enzyme TrCel7A was purified from enzyme mixture (Celluclast®). Fractionation of soluble cellooligosaccharides was performed using size exclusion chromatography (SEC) and hydrolysis of the cellooligosaccharides with degree of polymerisation (DP) from 3 to 6 was conducted by TrCel7A at 25 °C for 1 h, and the product concentration was analysed by highperformance liquid chromatography (HPLC). HPLC results showed that glucose was produced for each DP of cellooligosaccharides. However, the highest product that achieved for all DP of cellooligosaccharides was cellobiose, followed by cellotriose (in the case of DP 5 and 6) and glucose. The reaction of cellooligosaccharides with DP 3 to 6 during hydrolysis by TrCel7A were modelled to show exactly the cleavage sites for each DP of cellooligosaccharides. The model showed that all cellooligosaccharides was hydrolysed at the first (glucose) and second (cellobiose) glycosidic linkages by TrCel7A.
format Article
author Syed Abdul Rahman, Sharifah Annirah
Abang Zaidel, Dayang Norulfairuz
author_facet Syed Abdul Rahman, Sharifah Annirah
Abang Zaidel, Dayang Norulfairuz
author_sort Syed Abdul Rahman, Sharifah Annirah
title Fractionation and hydrolysis of cellooligosaccharides by Trichoderma reesei Cellobiohydrolase 7A
title_short Fractionation and hydrolysis of cellooligosaccharides by Trichoderma reesei Cellobiohydrolase 7A
title_full Fractionation and hydrolysis of cellooligosaccharides by Trichoderma reesei Cellobiohydrolase 7A
title_fullStr Fractionation and hydrolysis of cellooligosaccharides by Trichoderma reesei Cellobiohydrolase 7A
title_full_unstemmed Fractionation and hydrolysis of cellooligosaccharides by Trichoderma reesei Cellobiohydrolase 7A
title_sort fractionation and hydrolysis of cellooligosaccharides by trichoderma reesei cellobiohydrolase 7a
publisher Institute of Physics Publishing
publishDate 2020
url http://eprints.utm.my/id/eprint/87226/
http://dx.doi.org/10.1088/1757-899X/736/4/042022
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