Bioprocess optimization for exopolysaccharides production by ganoderma lucidum in semi-industrial scale
Background: For many years, Ganoderma was highly considered as biofactory for the production of different types of bioactive metabolites. Of these bioactive compounds, polysaccharides gained much attention based on their high biotherapeutic properties. Therefore, special attention has been paid duri...
Saved in:
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Published: |
Bentham Science Publishers
2020
|
Subjects: | |
Online Access: | http://eprints.utm.my/id/eprint/91758/ http://dx.doi.org/10.2174/2212798411666200316153148 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Universiti Teknologi Malaysia |
id |
my.utm.91758 |
---|---|
record_format |
eprints |
spelling |
my.utm.917582021-07-28T08:42:38Z http://eprints.utm.my/id/eprint/91758/ Bioprocess optimization for exopolysaccharides production by ganoderma lucidum in semi-industrial scale Abd. Alsaheb, Ramzy Koh, Zwin Zjeh Abd. Malek, Roslinda Abdullah, Jaafar Kamil El Baz, Ashraf El Deeb, Nehal Dailin, Daniel Hanapi, Siti Zulaiha Sukmawati, Dalia El Enshasy, Hesham TP Chemical technology Background: For many years, Ganoderma was highly considered as biofactory for the production of different types of bioactive metabolites. Of these bioactive compounds, polysaccharides gained much attention based on their high biotherapeutic properties. Therefore, special attention has been paid during the last years for the production of mushrooms bioactive compounds in a closed cultivation system to shorten the cultivation time and increase the product yield. Objectives: This work focuses on the development of a simple cultivation strategy for exopolysac-charides (EPS) production using Ganoderma lucidum and submerged cultivation system. Methods: At first, the best medium supporting EPS production was chosen experimentally from the current published data. Second, like many EPS production processes, carbon and nitrogen concen-trations were optimized to support the highest production of polysaccharides in the shake flask level. Furthermore, the process was scaled up in 16-L stirred tank bioreactor. Results: The results clearly demonstrated that the best cultivation strategy was cultivation under controlled pH conditions (pH 5.5). Under this condition, the maximal volumetric and specific yield of EPS production were, 5.0 g/L and 0.42 g/g, respectively. Conclusion: The current results clearly demonstrate the high potential use of submerged cultivation system as an alternative to conventional solid-state fermentation for EPS production by G. lucidum. Furthermore, the optimization of both carbon and nitrogen sources concentration and scaling up of the process showed a significant increase in both volumetric and specific EPS production. Bentham Science Publishers 2020 Article PeerReviewed Abd. Alsaheb, Ramzy and Koh, Zwin Zjeh and Abd. Malek, Roslinda and Abdullah, Jaafar Kamil and El Baz, Ashraf and El Deeb, Nehal and Dailin, Daniel and Hanapi, Siti Zulaiha and Sukmawati, Dalia and El Enshasy, Hesham (2020) Bioprocess optimization for exopolysaccharides production by ganoderma lucidum in semi-industrial scale. Recent Patents on Food, Nutrition and Agriculture, 11 (3). pp. 211-218. ISSN 2212-7984 http://dx.doi.org/10.2174/2212798411666200316153148 |
institution |
Universiti Teknologi Malaysia |
building |
UTM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Teknologi Malaysia |
content_source |
UTM Institutional Repository |
url_provider |
http://eprints.utm.my/ |
topic |
TP Chemical technology |
spellingShingle |
TP Chemical technology Abd. Alsaheb, Ramzy Koh, Zwin Zjeh Abd. Malek, Roslinda Abdullah, Jaafar Kamil El Baz, Ashraf El Deeb, Nehal Dailin, Daniel Hanapi, Siti Zulaiha Sukmawati, Dalia El Enshasy, Hesham Bioprocess optimization for exopolysaccharides production by ganoderma lucidum in semi-industrial scale |
description |
Background: For many years, Ganoderma was highly considered as biofactory for the production of different types of bioactive metabolites. Of these bioactive compounds, polysaccharides gained much attention based on their high biotherapeutic properties. Therefore, special attention has been paid during the last years for the production of mushrooms bioactive compounds in a closed cultivation system to shorten the cultivation time and increase the product yield. Objectives: This work focuses on the development of a simple cultivation strategy for exopolysac-charides (EPS) production using Ganoderma lucidum and submerged cultivation system. Methods: At first, the best medium supporting EPS production was chosen experimentally from the current published data. Second, like many EPS production processes, carbon and nitrogen concen-trations were optimized to support the highest production of polysaccharides in the shake flask level. Furthermore, the process was scaled up in 16-L stirred tank bioreactor. Results: The results clearly demonstrated that the best cultivation strategy was cultivation under controlled pH conditions (pH 5.5). Under this condition, the maximal volumetric and specific yield of EPS production were, 5.0 g/L and 0.42 g/g, respectively. Conclusion: The current results clearly demonstrate the high potential use of submerged cultivation system as an alternative to conventional solid-state fermentation for EPS production by G. lucidum. Furthermore, the optimization of both carbon and nitrogen sources concentration and scaling up of the process showed a significant increase in both volumetric and specific EPS production. |
format |
Article |
author |
Abd. Alsaheb, Ramzy Koh, Zwin Zjeh Abd. Malek, Roslinda Abdullah, Jaafar Kamil El Baz, Ashraf El Deeb, Nehal Dailin, Daniel Hanapi, Siti Zulaiha Sukmawati, Dalia El Enshasy, Hesham |
author_facet |
Abd. Alsaheb, Ramzy Koh, Zwin Zjeh Abd. Malek, Roslinda Abdullah, Jaafar Kamil El Baz, Ashraf El Deeb, Nehal Dailin, Daniel Hanapi, Siti Zulaiha Sukmawati, Dalia El Enshasy, Hesham |
author_sort |
Abd. Alsaheb, Ramzy |
title |
Bioprocess optimization for exopolysaccharides production by ganoderma lucidum in semi-industrial scale |
title_short |
Bioprocess optimization for exopolysaccharides production by ganoderma lucidum in semi-industrial scale |
title_full |
Bioprocess optimization for exopolysaccharides production by ganoderma lucidum in semi-industrial scale |
title_fullStr |
Bioprocess optimization for exopolysaccharides production by ganoderma lucidum in semi-industrial scale |
title_full_unstemmed |
Bioprocess optimization for exopolysaccharides production by ganoderma lucidum in semi-industrial scale |
title_sort |
bioprocess optimization for exopolysaccharides production by ganoderma lucidum in semi-industrial scale |
publisher |
Bentham Science Publishers |
publishDate |
2020 |
url |
http://eprints.utm.my/id/eprint/91758/ http://dx.doi.org/10.2174/2212798411666200316153148 |
_version_ |
1706956990016651264 |