Optimizing medium components to enhance high cell mass production of biotherapeutic strain lactobacillus reuteri DSM 20016T by statistical method

Optimizing medium components to enhance high cell mass production of biotherapeutic strain lactobacillus reuteri DSM 20016T by statistical method

Probiotics referred to a group of living microorganisms which highly influence the human health. A number of studies have highlighted on the bio-therapeutic potential of Lactobacillus reuteri strains, especially in treating eradication of Helicobacter pylori. Therefore, in present investigation, sta...

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Main Authors: Selvamani, S., Dailin, D. J., Rostom, M., A. Malek, R., Gupta, V. K., El-Enshasy, H. A.
Format: Article
Published: National Institute of Science Communication and Information Resources 2020
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TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/93262/
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Institution: Universiti Teknologi Malaysia
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spelling my.utm.932622021-11-19T03:29:52Z http://eprints.utm.my/id/eprint/93262/ Optimizing medium components to enhance high cell mass production of biotherapeutic strain lactobacillus reuteri DSM 20016T by statistical method Selvamani, S. Dailin, D. J. Rostom, M. A. Malek, R. Gupta, V. K. El-Enshasy, H. A. TP Chemical technology Probiotics referred to a group of living microorganisms which highly influence the human health. A number of studies have highlighted on the bio-therapeutic potential of Lactobacillus reuteri strains, especially in treating eradication of Helicobacter pylori. Therefore, in present investigation, statistical methods were applied to optimize medium composition for high cell mass production of L. reuteri strain DSM 20016T. Most influencing medium components were screened by using Plackett-Burman and optimized using Box-Bhenken experimental design. The concentration of lactose, yeast extract and phosphate in cultivation medium has shown significant effect on the cell mass production. The highest cell mass obtained after 48h incubation was 3.96 ± 0.02 gL−1 in RSM-optimized medium compared to 1.76 ± 0.17 gL−1 in un-optimized medium. National Institute of Science Communication and Information Resources 2020-09 Article PeerReviewed Selvamani, S. and Dailin, D. J. and Rostom, M. and A. Malek, R. and Gupta, V. K. and El-Enshasy, H. A. (2020) Optimizing medium components to enhance high cell mass production of biotherapeutic strain lactobacillus reuteri DSM 20016T by statistical method. Journal of Scientific and Industrial Research, 79 (9). pp. 798-803. ISSN 0022-4456
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Selvamani, S.
Dailin, D. J.
Rostom, M.
A. Malek, R.
Gupta, V. K.
El-Enshasy, H. A.
Optimizing medium components to enhance high cell mass production of biotherapeutic strain lactobacillus reuteri DSM 20016T by statistical method
description Probiotics referred to a group of living microorganisms which highly influence the human health. A number of studies have highlighted on the bio-therapeutic potential of Lactobacillus reuteri strains, especially in treating eradication of Helicobacter pylori. Therefore, in present investigation, statistical methods were applied to optimize medium composition for high cell mass production of L. reuteri strain DSM 20016T. Most influencing medium components were screened by using Plackett-Burman and optimized using Box-Bhenken experimental design. The concentration of lactose, yeast extract and phosphate in cultivation medium has shown significant effect on the cell mass production. The highest cell mass obtained after 48h incubation was 3.96 ± 0.02 gL−1 in RSM-optimized medium compared to 1.76 ± 0.17 gL−1 in un-optimized medium.
format Article
author Selvamani, S.
Dailin, D. J.
Rostom, M.
A. Malek, R.
Gupta, V. K.
El-Enshasy, H. A.
author_facet Selvamani, S.
Dailin, D. J.
Rostom, M.
A. Malek, R.
Gupta, V. K.
El-Enshasy, H. A.
author_sort Selvamani, S.
title Optimizing medium components to enhance high cell mass production of biotherapeutic strain lactobacillus reuteri DSM 20016T by statistical method
title_short Optimizing medium components to enhance high cell mass production of biotherapeutic strain lactobacillus reuteri DSM 20016T by statistical method
title_full Optimizing medium components to enhance high cell mass production of biotherapeutic strain lactobacillus reuteri DSM 20016T by statistical method
title_fullStr Optimizing medium components to enhance high cell mass production of biotherapeutic strain lactobacillus reuteri DSM 20016T by statistical method
title_full_unstemmed Optimizing medium components to enhance high cell mass production of biotherapeutic strain lactobacillus reuteri DSM 20016T by statistical method
title_sort optimizing medium components to enhance high cell mass production of biotherapeutic strain lactobacillus reuteri dsm 20016t by statistical method
publisher National Institute of Science Communication and Information Resources
publishDate 2020
url http://eprints.utm.my/id/eprint/93262/
_version_ 1717093443796205568

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