Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata

Ultrasound-mechanical stirrer assisted extraction (UMSAE) of extraction yield, total alkaloid content (TAC) and antioxidant (DPPH) activity from Annona muricata leaves were optimized using response surface methodology. Five factors were screened using Plackett-Burman Design to investigate the variab...

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Main Authors: Lee, C. H., Lee, T. H., Ong, P. Y., Wong, W. S., Hamdan, N., Elgharbawy, A. A. M., Azmi, N. A. A.
Format: Article
Published: Elsevier Ltd. 2021
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Online Access:http://eprints.utm.my/id/eprint/95301/
http://dx.doi.org/10.1016/j.procbio.2021.07.006
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Institution: Universiti Teknologi Malaysia
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spelling my.utm.953012022-04-29T22:03:20Z http://eprints.utm.my/id/eprint/95301/ Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata Lee, C. H. Lee, T. H. Ong, P. Y. Wong, W. S. Hamdan, N. Elgharbawy, A. A. M. Azmi, N. A. A. TP Chemical technology Ultrasound-mechanical stirrer assisted extraction (UMSAE) of extraction yield, total alkaloid content (TAC) and antioxidant (DPPH) activity from Annona muricata leaves were optimized using response surface methodology. Five factors were screened using Plackett-Burman Design to investigate the variables that significantly influence the extraction yield, TAC and DPPH activity, namely solvent concentration, extraction time, ultrasound power, plant material to solvent ratio, and extraction temperature. According to statistical significance analysis, solvent concentration, ultrasound power, plant material to solvent ratio, and extraction temperature were selected for further optimization of the UMSAE extraction process using a Central Composite Design. ANOVA results exhibited that the obtained models were significant at 95 % confidence level. Satisfactory extraction yields (26.38 %) and TAC (39.73 mg AE/g) were obtained with relatively short extraction time under the optimum conditions compared with conventional Soxhlet extraction. The extract prepared under UMSAE displayed excellent DPPH activity (93.15 %). The optimized UMSAE promoted considerable yield increases 1.59-fold the yield extract, 2.32-fold TAC, and 1.19-fold antioxidant activity if compared to the Soxhlet extraction. This study provides the ideal integrated UMSAE settings for the maximum recovery of total alkaloid content with high antioxidant activity from A. muricata for possible utilization of their leaves in food applications. Elsevier Ltd. 2021 Article PeerReviewed Lee, C. H. and Lee, T. H. and Ong, P. Y. and Wong, W. S. and Hamdan, N. and Elgharbawy, A. A. M. and Azmi, N. A. A. (2021) Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata. Process Biochemistry, 109 . pp. 104-116. ISSN 1359-5113 http://dx.doi.org/10.1016/j.procbio.2021.07.006 DOI: 10.1016/j.procbio.2021.07.006
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Lee, C. H.
Lee, T. H.
Ong, P. Y.
Wong, W. S.
Hamdan, N.
Elgharbawy, A. A. M.
Azmi, N. A. A.
Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata
description Ultrasound-mechanical stirrer assisted extraction (UMSAE) of extraction yield, total alkaloid content (TAC) and antioxidant (DPPH) activity from Annona muricata leaves were optimized using response surface methodology. Five factors were screened using Plackett-Burman Design to investigate the variables that significantly influence the extraction yield, TAC and DPPH activity, namely solvent concentration, extraction time, ultrasound power, plant material to solvent ratio, and extraction temperature. According to statistical significance analysis, solvent concentration, ultrasound power, plant material to solvent ratio, and extraction temperature were selected for further optimization of the UMSAE extraction process using a Central Composite Design. ANOVA results exhibited that the obtained models were significant at 95 % confidence level. Satisfactory extraction yields (26.38 %) and TAC (39.73 mg AE/g) were obtained with relatively short extraction time under the optimum conditions compared with conventional Soxhlet extraction. The extract prepared under UMSAE displayed excellent DPPH activity (93.15 %). The optimized UMSAE promoted considerable yield increases 1.59-fold the yield extract, 2.32-fold TAC, and 1.19-fold antioxidant activity if compared to the Soxhlet extraction. This study provides the ideal integrated UMSAE settings for the maximum recovery of total alkaloid content with high antioxidant activity from A. muricata for possible utilization of their leaves in food applications.
format Article
author Lee, C. H.
Lee, T. H.
Ong, P. Y.
Wong, W. S.
Hamdan, N.
Elgharbawy, A. A. M.
Azmi, N. A. A.
author_facet Lee, C. H.
Lee, T. H.
Ong, P. Y.
Wong, W. S.
Hamdan, N.
Elgharbawy, A. A. M.
Azmi, N. A. A.
author_sort Lee, C. H.
title Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata
title_short Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata
title_full Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata
title_fullStr Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata
title_full_unstemmed Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata
title_sort integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata
publisher Elsevier Ltd.
publishDate 2021
url http://eprints.utm.my/id/eprint/95301/
http://dx.doi.org/10.1016/j.procbio.2021.07.006
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