A novel approach in lactose based induction for enhanced production of 1-4-beta xylanase by recombinant Escherichia coli

Escherichia coli BL21 (DE3) with a plasmid vector pET-22b (+) carrying xylanase coding gene isolated from an extremely thermophilic bacterium, Thermotoga sp. was used in this work for enhanced production of recombinant xylanase. The study was focused on optimization of lactose based induction for en...

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Main Authors: Kandiyil, Subeesh Kunhi, Malek, R. A., Kaul Hatti, R., Ho, C. K., El Enshasy, H. E.
Format: Article
Published: National Institute of Science Communication and Information Resources 2019
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Online Access:http://eprints.utm.my/id/eprint/96956/
http://nopr.niscpr.res.in/handle/123456789/47155
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spelling my.utm.969562022-09-04T07:53:01Z http://eprints.utm.my/id/eprint/96956/ A novel approach in lactose based induction for enhanced production of 1-4-beta xylanase by recombinant Escherichia coli Kandiyil, Subeesh Kunhi Malek, R. A. Kaul Hatti, R. Ho, C. K. El Enshasy, H. E. TP Chemical technology Escherichia coli BL21 (DE3) with a plasmid vector pET-22b (+) carrying xylanase coding gene isolated from an extremely thermophilic bacterium, Thermotoga sp. was used in this work for enhanced production of recombinant xylanase. The study was focused on optimization of lactose based induction for enhanced production of xylanase. Data collected from OFAT and DoE based trails indicated that initiation of lactose based induction at the very early phase of cell growth in maltose base media enhances xylanase production significantly. During further induction strategy optimization trails, intracellular xylanase production was enhanced up to 2600 IU mL-1 when the expression was induced by 8 gL-1 of lactose at early log phase (between 2-4 hours) of the cell growth. From induction temperature optimization trials, it was found that xylanase production can be further enhanced by reducing the incubation temperature from 37 to 32ºC. Using the optimum induction strategy developed, up to 6430 IU mL-1 xylanase activity was achieved. This value was almost 3 folds higher than those obtained in IPTG induced cultures. National Institute of Science Communication and Information Resources 2019 Article PeerReviewed Kandiyil, Subeesh Kunhi and Malek, R. A. and Kaul Hatti, R. and Ho, C. K. and El Enshasy, H. E. (2019) A novel approach in lactose based induction for enhanced production of 1-4-beta xylanase by recombinant Escherichia coli. Journal of Scientific and Industrial Research, 78 (5). pp. 287-294. ISSN 0022-4456 (In Press) http://nopr.niscpr.res.in/handle/123456789/47155 NA
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Kandiyil, Subeesh Kunhi
Malek, R. A.
Kaul Hatti, R.
Ho, C. K.
El Enshasy, H. E.
A novel approach in lactose based induction for enhanced production of 1-4-beta xylanase by recombinant Escherichia coli
description Escherichia coli BL21 (DE3) with a plasmid vector pET-22b (+) carrying xylanase coding gene isolated from an extremely thermophilic bacterium, Thermotoga sp. was used in this work for enhanced production of recombinant xylanase. The study was focused on optimization of lactose based induction for enhanced production of xylanase. Data collected from OFAT and DoE based trails indicated that initiation of lactose based induction at the very early phase of cell growth in maltose base media enhances xylanase production significantly. During further induction strategy optimization trails, intracellular xylanase production was enhanced up to 2600 IU mL-1 when the expression was induced by 8 gL-1 of lactose at early log phase (between 2-4 hours) of the cell growth. From induction temperature optimization trials, it was found that xylanase production can be further enhanced by reducing the incubation temperature from 37 to 32ºC. Using the optimum induction strategy developed, up to 6430 IU mL-1 xylanase activity was achieved. This value was almost 3 folds higher than those obtained in IPTG induced cultures.
format Article
author Kandiyil, Subeesh Kunhi
Malek, R. A.
Kaul Hatti, R.
Ho, C. K.
El Enshasy, H. E.
author_facet Kandiyil, Subeesh Kunhi
Malek, R. A.
Kaul Hatti, R.
Ho, C. K.
El Enshasy, H. E.
author_sort Kandiyil, Subeesh Kunhi
title A novel approach in lactose based induction for enhanced production of 1-4-beta xylanase by recombinant Escherichia coli
title_short A novel approach in lactose based induction for enhanced production of 1-4-beta xylanase by recombinant Escherichia coli
title_full A novel approach in lactose based induction for enhanced production of 1-4-beta xylanase by recombinant Escherichia coli
title_fullStr A novel approach in lactose based induction for enhanced production of 1-4-beta xylanase by recombinant Escherichia coli
title_full_unstemmed A novel approach in lactose based induction for enhanced production of 1-4-beta xylanase by recombinant Escherichia coli
title_sort novel approach in lactose based induction for enhanced production of 1-4-beta xylanase by recombinant escherichia coli
publisher National Institute of Science Communication and Information Resources
publishDate 2019
url http://eprints.utm.my/id/eprint/96956/
http://nopr.niscpr.res.in/handle/123456789/47155
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