Enhancement biodegradation of n-alkanes from crude oil contaminated seawater

The aim of this research was to optimize bioremediation of seawater samples spiked with 1000 mg/L crude oil for removal of n-alkanes (C12H26 to C34H70). Bioaugmentation experiments were performed at laboratory scale: each bioreactor contained 250 ml dispersed crude-oil-contaminated seawater, indigen...

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Main Authors: Zahed, M.A., Aziz, H.A., Isa, M.H., Mohajeri, L.
Format: Citation Index Journal
Published: 2010
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Online Access:http://eprints.utp.edu.my/6467/1/Enhancement_biodegradation_of_n-alkanes_from_crude_oil_contaminated_seawater.pdf
http://eprints.utp.edu.my/6467/
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spelling my.utp.eprints.64672017-03-20T01:59:48Z Enhancement biodegradation of n-alkanes from crude oil contaminated seawater Zahed, M.A. Aziz, H.A. Isa, M.H. Mohajeri, L. TD Environmental technology. Sanitary engineering The aim of this research was to optimize bioremediation of seawater samples spiked with 1000 mg/L crude oil for removal of n-alkanes (C12H26 to C34H70). Bioaugmentation experiments were performed at laboratory scale: each bioreactor contained 250 ml dispersed crude-oil-contaminated seawater, indigenous acclimatized microorganism and nitrogen and phosphorus at concentrations based on central composite design (CCD) calculations. Three independent variables, time, nitrogen and phosphorus, were investigated and the experimental data obtained were fitted to a second-order polynomial mathematical model with multiple regressions. The obtained Model F-value of 97.12 and probability F <0.0001 implies the model is significant. Hydrocarbon analyses were carried out using a gas chromatograph equipped with flame ionization detector (GC/FID). During 28 days of experimentation, a maximum of 85.35% total n-alkane removal was observed. Numerical optimization was achieved based on desirability functions. Using 188.71 mg/L nitrogen and 18.99 mg/L phosphorus, design of experiment (DOE) software predicted 91.00% removal. A removal of 92.04% was observed experimentally, in close agreement with the predicted value. 2010-12 Citation Index Journal PeerReviewed application/pdf http://eprints.utp.edu.my/6467/1/Enhancement_biodegradation_of_n-alkanes_from_crude_oil_contaminated_seawater.pdf Zahed, M.A. and Aziz, H.A. and Isa, M.H. and Mohajeri, L. (2010) Enhancement biodegradation of n-alkanes from crude oil contaminated seawater. [Citation Index Journal] http://eprints.utp.edu.my/6467/
institution Universiti Teknologi Petronas
building UTP Resource Centre
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Petronas
content_source UTP Institutional Repository
url_provider http://eprints.utp.edu.my/
topic TD Environmental technology. Sanitary engineering
spellingShingle TD Environmental technology. Sanitary engineering
Zahed, M.A.
Aziz, H.A.
Isa, M.H.
Mohajeri, L.
Enhancement biodegradation of n-alkanes from crude oil contaminated seawater
description The aim of this research was to optimize bioremediation of seawater samples spiked with 1000 mg/L crude oil for removal of n-alkanes (C12H26 to C34H70). Bioaugmentation experiments were performed at laboratory scale: each bioreactor contained 250 ml dispersed crude-oil-contaminated seawater, indigenous acclimatized microorganism and nitrogen and phosphorus at concentrations based on central composite design (CCD) calculations. Three independent variables, time, nitrogen and phosphorus, were investigated and the experimental data obtained were fitted to a second-order polynomial mathematical model with multiple regressions. The obtained Model F-value of 97.12 and probability F <0.0001 implies the model is significant. Hydrocarbon analyses were carried out using a gas chromatograph equipped with flame ionization detector (GC/FID). During 28 days of experimentation, a maximum of 85.35% total n-alkane removal was observed. Numerical optimization was achieved based on desirability functions. Using 188.71 mg/L nitrogen and 18.99 mg/L phosphorus, design of experiment (DOE) software predicted 91.00% removal. A removal of 92.04% was observed experimentally, in close agreement with the predicted value.
format Citation Index Journal
author Zahed, M.A.
Aziz, H.A.
Isa, M.H.
Mohajeri, L.
author_facet Zahed, M.A.
Aziz, H.A.
Isa, M.H.
Mohajeri, L.
author_sort Zahed, M.A.
title Enhancement biodegradation of n-alkanes from crude oil contaminated seawater
title_short Enhancement biodegradation of n-alkanes from crude oil contaminated seawater
title_full Enhancement biodegradation of n-alkanes from crude oil contaminated seawater
title_fullStr Enhancement biodegradation of n-alkanes from crude oil contaminated seawater
title_full_unstemmed Enhancement biodegradation of n-alkanes from crude oil contaminated seawater
title_sort enhancement biodegradation of n-alkanes from crude oil contaminated seawater
publishDate 2010
url http://eprints.utp.edu.my/6467/1/Enhancement_biodegradation_of_n-alkanes_from_crude_oil_contaminated_seawater.pdf
http://eprints.utp.edu.my/6467/
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