Sensitive Detection and Quantification of Burkholderia pseudomallei in Soil Samples Using Culture-Dependent and-Independent Approaches

• Main Authors: Trinh, Thanh Trung, Steinmetz, Ivo, Hetzer, Adrian
• Format: Working Paper
• Language: English
• Published: NXB Nông Nghiệp 2011
• Subjects: Burkholderia pseudomallei; meliodosis; polyethylene glycol; sodium deoxycholate; culture-dependent method; culture-independent method
• Online Access: http://repository.vnu.edu.vn/handle/123456789/8934
• Institution: Vietnam National University, Hanoi

Burkholderia pseudomallei, the causative agent of melioidosis, is a soil-dwelling Gram-negative bacterium. Although the bacterium has been known for a century, its worldwide distribution as well as its natural lifestyle still remains uncertain. Detection of B. pseudomalleiusing culture-dependent and-independent methods has been previously reported. However, these methods have demonstrated either lack sensitivity or inaccurate quantification; therefore, environmental samples containing low numbers of B. pseudomalleiwill likely remain undetected or unquantifiable