Ethanol extracts of Scutellaria baicalensisprotect against lipopolysaccharide-induced acute liver injury in mice

• Main Authors: Nguyen, Thanh Hai, Le, Tuan Anh, Duong, Thi Ly Huong, Nguyen, Huu Tung, Vu, Duc Loi, Dang, Thu Kim, Bui, Thanh Tung
• Format: Article
• Language: English
• Published: Elsevier 2016
• Subjects: Scutellaria baicalensis; Lipopolysaccharide; Liver injury; Cytokine; Nuclear factor-κB
• Online Access: http://repository.vnu.edu.vn/handle/VNU_123/11455
• Institution: Vietnam National University, Hanoi

Objective: To investigated the protective potential of ethanol extracts of Scutellaria baicalensis (S. baicalensis) against lipopolysaccharide (LPS)-induced liver injury. Methods:Dried roots of S. baicalensiswere extracted with ethanol and concentrated to yield a dry residue. Mice were administered 200 mg/kg of the ethanol extracts orally once daily for one week. Animals were subsequently administered a single dose of LPS(5 mg/kg of body weight, intraperitoneal injection). Both protein and mRNAlevels of cytokines, such as tumor necrosis factor alpha, interleukin-1β, and interleukin-6 in liver tissues were evaluated by ELISA assay and quantitative PCR. Cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB protein levels in liver tissues were analyzed by western blotting. Results:Liver injury induced by LPSsignificantly increased necrosis factor alpha, interleukin-1β, interleukin-6, cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB in liver tissues. Treatment with ethanol extracts of S. baicalensisprevented all of these observed changes associated with LPS-induced injury in liver mice. Conclusions:Our study showed that S. baicalensisis potentially protective against LPS-induced liver injury in mice