Construction of Yeast Pichia pastoris Expressing the Recombinant p53 as a Secreted Protein into Culture
p. 336-343
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2018
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oai:112.137.131.14:VNU_123-615542018-04-26T20:06:03Z Construction of Yeast Pichia pastoris Expressing the Recombinant p53 as a Secreted Protein into Culture Tran, Thi Thuy Nga Nguyen, Quang Hoa Hoang, Van Tuyen Do, Thi Tuyen Dinh, Nho Thai Pichia pastoris, gene expression p53, codon optimization p. 336-343 Human p53 protein has been known as a tumor suppressor and described as "the guardian of the genome", referring to its role in conserving stability of genome by preventing mutation. The recent studies on p53 protein expression have demonstrated the important role and effectiveness of exogenous p53 protein in tumor suppression. In the world, the expression of p53 for therapeutically interest was extensively study but in Vietnam it has not been noticed. In the other hand, Pichia pastoris showed a good expression system for many exogenous proteins with a simple cloning work and cheap culture. In this study, codon optimization of gene encoding human p53 protein was performed for suitable expression in Pichia pastoris yeast. It was designed as construct of p53 fused with TAT and His-tag sequences (TAT-p53-His). The construct was cloned into pPICZαA expression vector by using EcoRI and XbaI enzymes to make pPICZαA-TAT-p53-His. Yeast strains containing genes coding for TAT-p53-His was obtained. The integration of TAT-p53-His construct into yeast genome was verified by using PCR with AOX1 primers. The expression of the recombinant TAT-p53-His in the culture of P. pastoris X33 was confirmed by SDS-PAGE. 2018-03-30T02:40:58Z 2018-03-30T02:40:58Z 2016 Article 2588-1140. http://repository.vnu.edu.vn/handle/VNU_123/61554 other Vol 32;No 1S application/pdf H. : ĐHQGHN |
| institution |
Vietnam National University, Hanoi |
| building |
VNU Library & Information Center |
| country |
Vietnam |
| collection |
VNU Digital Repository |
| language |
other |
| topic |
Pichia pastoris, gene expression p53, codon optimization |
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Pichia pastoris, gene expression p53, codon optimization Tran, Thi Thuy Nga Nguyen, Quang Hoa Hoang, Van Tuyen Do, Thi Tuyen Dinh, Nho Thai Construction of Yeast Pichia pastoris Expressing the Recombinant p53 as a Secreted Protein into Culture |
| description |
p. 336-343 |
| format |
Article |
| author |
Tran, Thi Thuy Nga Nguyen, Quang Hoa Hoang, Van Tuyen Do, Thi Tuyen Dinh, Nho Thai |
| author_facet |
Tran, Thi Thuy Nga Nguyen, Quang Hoa Hoang, Van Tuyen Do, Thi Tuyen Dinh, Nho Thai |
| author_sort |
Tran, Thi Thuy Nga |
| title |
Construction of Yeast Pichia pastoris Expressing the Recombinant p53 as a Secreted Protein into Culture |
| title_short |
Construction of Yeast Pichia pastoris Expressing the Recombinant p53 as a Secreted Protein into Culture |
| title_full |
Construction of Yeast Pichia pastoris Expressing the Recombinant p53 as a Secreted Protein into Culture |
| title_fullStr |
Construction of Yeast Pichia pastoris Expressing the Recombinant p53 as a Secreted Protein into Culture |
| title_full_unstemmed |
Construction of Yeast Pichia pastoris Expressing the Recombinant p53 as a Secreted Protein into Culture |
| title_sort |
construction of yeast pichia pastoris expressing the recombinant p53 as a secreted protein into culture |
| publisher |
H. : ĐHQGHN |
| publishDate |
2018 |
| url |
http://repository.vnu.edu.vn/handle/VNU_123/61554 |
| _version_ |
1680966246153060352 |