Establishment of an in vitro screening model of bioactive compounds from Vietnamese medicinal plants using the recombinant central nervous system receptors
p. 105-110
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2018
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oai:112.137.131.14:VNU_123-616232018-04-26T20:09:45Z Establishment of an in vitro screening model of bioactive compounds from Vietnamese medicinal plants using the recombinant central nervous system receptors Xây dựng mô hình sàng lọc in vitro hoạt chất hướng đích dựa trên các thụ thể hệ thần kinh trung ương tái tổ hợp Pham, Thi Hong Nhung Dinh, Doan Long Recombinant CNS receptors an in vitro screening assays bioactive compounds Semliki Forest virus specificity p. 105-110 The central nervous system receptors are important targets of the drugs, involved in many neurological diseases. Therefore, this study was designed to build an in vitro screening model using recombinant receptors distributed in the central nervous system (CNS). Method: construction of cDNA system encoding for receptors; using Semliki Forest virus for the rapid and high expression of receptors in mammalian cell lines; designing binding assays for in vitro pharmacological studies of compounds and methanol plant extracts. Results: 24 cDNAs encoding for receptors and 1 screening kit with neurokinin-1 receptor were constructed; 4 receptors were expressed successfully. Conclusion: The in vitro screening model was established successfully and applied for NK1 receptors with high sensitivity and specificity. This model is a useful tool for discovery and development of target compounds acting in the CNS 2018-04-24T02:21:27Z 2018-04-24T02:21:27Z 2018 Article 2588-1140 http://repository.vnu.edu.vn/handle/VNU_123/61623 other Vol 34;No 1 application/pdf H. : ĐHQGHN |
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Vietnam National University, Hanoi |
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VNU Library & Information Center |
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Vietnam |
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VNU Digital Repository |
| language |
other |
| topic |
Recombinant CNS receptors an in vitro screening assays bioactive compounds Semliki Forest virus specificity |
| spellingShingle |
Recombinant CNS receptors an in vitro screening assays bioactive compounds Semliki Forest virus specificity Pham, Thi Hong Nhung Dinh, Doan Long Establishment of an in vitro screening model of bioactive compounds from Vietnamese medicinal plants using the recombinant central nervous system receptors |
| description |
p. 105-110 |
| format |
Article |
| author |
Pham, Thi Hong Nhung Dinh, Doan Long |
| author_facet |
Pham, Thi Hong Nhung Dinh, Doan Long |
| author_sort |
Pham, Thi Hong Nhung |
| title |
Establishment of an in vitro screening model of bioactive compounds from Vietnamese medicinal plants using the recombinant central nervous system receptors |
| title_short |
Establishment of an in vitro screening model of bioactive compounds from Vietnamese medicinal plants using the recombinant central nervous system receptors |
| title_full |
Establishment of an in vitro screening model of bioactive compounds from Vietnamese medicinal plants using the recombinant central nervous system receptors |
| title_fullStr |
Establishment of an in vitro screening model of bioactive compounds from Vietnamese medicinal plants using the recombinant central nervous system receptors |
| title_full_unstemmed |
Establishment of an in vitro screening model of bioactive compounds from Vietnamese medicinal plants using the recombinant central nervous system receptors |
| title_sort |
establishment of an in vitro screening model of bioactive compounds from vietnamese medicinal plants using the recombinant central nervous system receptors |
| publisher |
H. : ĐHQGHN |
| publishDate |
2018 |
| url |
http://repository.vnu.edu.vn/handle/VNU_123/61623 |
| _version_ |
1680963570757533696 |