A system study on the effect of inductively coupled electrical stimulation (ICES) on hair growth cycle of Sprague Dawley rats

• Main Authors: Beltran, Matthew Joachim M., Chiu, John Lemuel L., Permentilla, Markson Kim L.
• Format: text
• Language: English
• Published: Animo Repository 2017
• Subjects: Rats as laboratory animals
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/9879
• Institution: De La Salle University

The in vivo effects of inductively coupled electrical simulation (ICES) device on the hair growth area, follicular density, and follicular morphology of Sprague Dawley rats were studied and demonstrated during 17-day observation duration. A treated and a control group, which consisted of 5 rats per group, were observed for 17 days. The rats were observed following parameters of hair growth: area, follicular density, and follicular morphology. For hair growth area, it was measured via image software analysis using ImageJ. For the follicular density and morphology, a histopathological analysis was done. Statistical analysis was done after collecting the data gathered and observed from the experiment. The following tests were done, T-test ANOVA, percentage difference, and test of difference, in order to test the effectiveness of ICES on hair growth area, follicular density, and follicular morphology on Sprague-Dawley rats. Based on the results of the analysis, the researchers were able to conclude that the asymmetric and biphasic waveform emitted by the ICES digiceutical device was effective in the observation greater follicular density. The treated group was observed to have a greater follicular density as observed from histopathological analysis. Furthermore, the researchers found that for every 20 follicles, the experimental group had more hair that were actively growing (Anagen) with an average 11.8 follicles vs. the control's 7.8. This was further proven by a p-value of 0.0005. Moreover, there were more hair in the resting phase (Telogen) for the experimental group as well, with 6.4 follicles as compared to the control group's 4.2 with a p-value of 0.049. Findings of the overall area growth measurement after 17 days did not show a significant difference between the two groups of rats, with a p value of 0.90. It was found out that there was no significant difference that existed between the experimental and control groups per week, the p-value retrieved was 0.095 which was greater than the alpha 0.05. The study concluded that treatment using the ICES device had a significant increase in the number of hairs that were in the growing stage of the hair growth cycle. Furthermore, the researchers were also able to prove that the follicular density of the treated rats was significantly greater as compared to the control group.