Antibiotic sensitivity patterns and enterotoxigenicity of Escherichia coli isolated from watery and formed stools of swines from Pasig City slaughterhouse

• Main Authors: de la Cerna, Elieza Marie A., Gallego, Marie-Lou L., Jiao, Eric Jason L., Perez, Ryan D.
• Format: text
• Language: English
• Published: Animo Repository 1999
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/10162
• Institution: De La Salle University

A total of thirty E. coli isolates were obtained from fecal samples of swines in Pasig City Slaughterhouse. Fifteen of the thirty isolates were derived from dry stools while the other half were obtained from wet stools. The identity of E. coli isolates were verified using triple sugar iron agar (TSI), Indole production Methyl-red test Vogues-Proskauer test Citrate utilization (IMViC), and gram staining. The enterotoxin production of the isolate was determined using the infant mouse bioassay. All the fifteen E. coli isolates from formed stools resulted negative for enterotoxin production. Out of the fifteen E. coli isolates from wet stools, three (20%) exhibited strongly positive, eleven (73.3%) showed slightly positive and only one (6.7%) exhibited for enterotoxin production. Results of antibiotic susceptibility patterns showed that all of the E. coli isolates from watery and formed stools were susceptible to the antibiotic neomycin. Out of fifteen E. coli isolates from formed stools, three (20%) showed susceptibility to amoxycillin. Out of fifteen isolates from wet stool samples, five (33.3%) showed susceptible, one (6.7%) intermediate and nine (60%) resistant to amoxycillin. All isolates were resistant to penicillin G, trimethoprim, tetracycline and oxytetracycline.