Effect of particle size, solvent, and extraction time on tannin extract from spondias purpurea bark through soxhlet extraction

Tannis are phytochemical polyphenols that have wide range of uses in industries such as the leather tanning, textile dyeing, ink, wood adhesive, and wine industries. Their many uses make them significant and in demand in various industries. Throughout time, many different trees have been studied to...

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Main Authors: Baldosano, Hilary Y., Castillo, Ma. Beatriz Micaela G., Elloran, Chantal Danica H.
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Language:English
Published: Animo Repository 2014
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Online Access:https://animorepository.dlsu.edu.ph/etd_bachelors/11409
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Institution: De La Salle University
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spelling oai:animorepository.dlsu.edu.ph:etd_bachelors-120542022-03-10T01:14:16Z Effect of particle size, solvent, and extraction time on tannin extract from spondias purpurea bark through soxhlet extraction Baldosano, Hilary Y. Castillo, Ma. Beatriz Micaela G. Elloran, Chantal Danica H. Tannis are phytochemical polyphenols that have wide range of uses in industries such as the leather tanning, textile dyeing, ink, wood adhesive, and wine industries. Their many uses make them significant and in demand in various industries. Throughout time, many different trees have been studied to determine their tannin content. In this study, the tannin content of Spondias purpurea or siniguelas bark was explored due to the lack of information and studies on the species. This study aimed to investigate the effects of particle size, solvent type and extraction time on the extraction of tannin from its bark through Soxhlet extraction. The ratios of solvent used were ethanol-water solutions at 0:1, 1:1, and 0.5:9.5. The extraction time was also varied at 4, 6, and 8 hours. The extracts were then analyzed using a Perkin Elmer High-Performance Liquid Chromatography (HPLC) with UV/VIS detector. The HPLC was performed using a reversed phase C-18 column as the stationary phase, and methanol (Solvent A) and 1:25 aqueous acetic acid solution (Solvent B) as the mobile phase. It was confirmed that extraction parameters varied in this study affected the yield of tannin in the extract. Finer particle sizes can produce a higher yield of tannin as long as agglomeration in the extractor does not occur. Moreover, a longer extraction time produces a higher yield. Solvents with higher amount of ethanol gave better yields of tannin. Pure water, as a solvent, gave the lowest amounts of tannin due to the solubility of proteins the 95% ethanol was the most effective. It was observed that 95% ethanol with the longest extraction time of 8 hours gave the highest percentage yield of 19.19% and 17.13% from 10 grams of bark. The model generated that gave an insignificant lack of fit was quadratic. Using ANOVA, the P-value obtained from the model was less than 0.0001, 2014-01-01T08:00:00Z text https://animorepository.dlsu.edu.ph/etd_bachelors/11409 Bachelor's Theses English Animo Repository Tannin plants Tanning Hides and skins Chemical Engineering
institution De La Salle University
building De La Salle University Library
continent Asia
country Philippines
Philippines
content_provider De La Salle University Library
collection DLSU Institutional Repository
language English
topic Tannin plants
Tanning
Hides and skins
Chemical Engineering
spellingShingle Tannin plants
Tanning
Hides and skins
Chemical Engineering
Baldosano, Hilary Y.
Castillo, Ma. Beatriz Micaela G.
Elloran, Chantal Danica H.
Effect of particle size, solvent, and extraction time on tannin extract from spondias purpurea bark through soxhlet extraction
description Tannis are phytochemical polyphenols that have wide range of uses in industries such as the leather tanning, textile dyeing, ink, wood adhesive, and wine industries. Their many uses make them significant and in demand in various industries. Throughout time, many different trees have been studied to determine their tannin content. In this study, the tannin content of Spondias purpurea or siniguelas bark was explored due to the lack of information and studies on the species. This study aimed to investigate the effects of particle size, solvent type and extraction time on the extraction of tannin from its bark through Soxhlet extraction. The ratios of solvent used were ethanol-water solutions at 0:1, 1:1, and 0.5:9.5. The extraction time was also varied at 4, 6, and 8 hours. The extracts were then analyzed using a Perkin Elmer High-Performance Liquid Chromatography (HPLC) with UV/VIS detector. The HPLC was performed using a reversed phase C-18 column as the stationary phase, and methanol (Solvent A) and 1:25 aqueous acetic acid solution (Solvent B) as the mobile phase. It was confirmed that extraction parameters varied in this study affected the yield of tannin in the extract. Finer particle sizes can produce a higher yield of tannin as long as agglomeration in the extractor does not occur. Moreover, a longer extraction time produces a higher yield. Solvents with higher amount of ethanol gave better yields of tannin. Pure water, as a solvent, gave the lowest amounts of tannin due to the solubility of proteins the 95% ethanol was the most effective. It was observed that 95% ethanol with the longest extraction time of 8 hours gave the highest percentage yield of 19.19% and 17.13% from 10 grams of bark. The model generated that gave an insignificant lack of fit was quadratic. Using ANOVA, the P-value obtained from the model was less than 0.0001,
format text
author Baldosano, Hilary Y.
Castillo, Ma. Beatriz Micaela G.
Elloran, Chantal Danica H.
author_facet Baldosano, Hilary Y.
Castillo, Ma. Beatriz Micaela G.
Elloran, Chantal Danica H.
author_sort Baldosano, Hilary Y.
title Effect of particle size, solvent, and extraction time on tannin extract from spondias purpurea bark through soxhlet extraction
title_short Effect of particle size, solvent, and extraction time on tannin extract from spondias purpurea bark through soxhlet extraction
title_full Effect of particle size, solvent, and extraction time on tannin extract from spondias purpurea bark through soxhlet extraction
title_fullStr Effect of particle size, solvent, and extraction time on tannin extract from spondias purpurea bark through soxhlet extraction
title_full_unstemmed Effect of particle size, solvent, and extraction time on tannin extract from spondias purpurea bark through soxhlet extraction
title_sort effect of particle size, solvent, and extraction time on tannin extract from spondias purpurea bark through soxhlet extraction
publisher Animo Repository
publishDate 2014
url https://animorepository.dlsu.edu.ph/etd_bachelors/11409
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