The analgesic effect of a mixture of Bauerenol, A-Amyrin and B-Amyrin in A 1:1:1 ratio from Ardisia SP. Leaves on ICR mice

• Main Authors: Evangelista, Juan Carlo, Kim, Sang Hyeon, Lo, Marvin James A.
• Format: text
• Language: English
• Published: Animo Repository 2011
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/11814
• Institution: De La Salle University

Ardisia sp. is a genus plants found in tropical and subtropical areas has been known for their phytoparmacheutical compounds with various properties, testing them for helpful bioactivities lead to the discovery of new medicines which help treat millions of people. A mixture of bauerenol, Ü-amyrin, Ý-amyrin in a 1:1:1 ratio was evaluated for analgesic bioactivity. Three bioassays were used: acetic acid writhing assay, hotplate assay and tailflick assay. The hotplate and tailflick assays test the compound’s inhibition of sensitivity to thermally induced pain. Both were performed at two points post administration (30 and 75 minutes). The acetic acid writhing assay tests the compounds inhibition of sensitivity to chemically induced pain. The acetic acid writhing assay is an observation of the test animals for 10 minutes post injection of the acetic acid. The compound did not produce an analgesic effect in the hotplate and tailflick assays which test for thermally induced pain, however, the data collected in the acetic acid writhing assay suggests a possible inhibition of the chemically induced pain.