Antimicrobial activity of compounds from Ardisia sp. (Family myrsinaceae) and Moringa oleifera

• Main Authors: Fernando, Katerina Mae F., San Pedro, Allison Marie R., Velasco, Hannah Camille D.
• Format: text
• Language: English
• Published: Animo Repository 2011
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/12125
• Institution: De La Salle University

Four compounds, Asp1 and Asp7 from Ardisia sp. and Mos1 and Mos8 from Moringa oleifera were tested for their antimicrobial activity against Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA) and Candida albicans. Both Asp1 and Asp7 are triterpenes in which Asp1 is composed of the three compounds Ü-amyrin, Ý- amyrin and bauerenol while ursenone, oleanone and baurenone constitute Asp7. Mos1 and Mos8 have similar primary structures differing only in their functional groups. The minimum inhibitory concentrations of each compound for the microbial isolates were determined using the well-diffusion assay. The study showed that the MIC of Asp1 for B. subtilis, MSSA and MRSA were 0.2 og oL-1, 2 og oL-1 and 5 og oL-1 respectively. While the MIC of Mos8 was 500 og oL-1 for bacteria B. subtilis and MSSA. Mos1 was only able to inhibit B. subtilis at a MIC of 500 og oL-1 . Asp7 did not inhibit any of the test microorganism even at 500 og oL-1 , the highest concentration tested. Escherichia coli, Klebsiella pneumonia and Candida albicans were not inhibited by any of the compounds tested. The compound Asp1 showed the most antimicrobial activity and this could be attributed to the hydroxyl groups present in its composition.