Cytotoxic activity of extracts from Philippine endemic plants Basella alba, Ipomoea batatas, and Mimosa pudica on human cancer cell lines

• Main Authors: Hinolan, Sean Francis E., Salting, Alexandra J.
• Format: text
• Language: English
• Published: Animo Repository 2017
• Subjects: Basellaceae; Sweet potatoes; Sensitive plant; Cancer cells; Cell death; Materia medica—Philippines; Biology
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/18689
• Institution: De La Salle University

This study determined the cytotoxicity of methanolic and ethanolic extracts from alugbati (Basella alba), kamote (Ipomoea batatas), and makahiya (Mimosa pudica) plants on cancer cell lines and normal cell lines. The extracts were obtained by macerating fresh weights of the plants with methanol or ethanol. This was followed by the removal of the solvent using rotary vacuum evaporation. The phosphate buffered saline- reconstituted extracts were filter—sterilized and their cytotoxicity was determined on various cell lines. These were the colon cancer cell lines-HTCC-116 and HT-29-, human small cell lung cancer cell line(H69PR), and the normal hepatocytes THLE-3 using PrestoBlue® resazurin assay. The chemotherapeutic drug Zeocin was used as positive control. Cytotoxicity tests showed that all plant extracts were highly cytotoxic to the cancer and normal cell lines as evidenced by their IC50 values. No significant differences were found between the treatments and positive control.The results showed that despite their high cytotoxicity on cancer cells, the use of the extracts as chemotherapeutic drugs has their limitation as they may affect the body negatively primarily because of their cytotoxicity on the normal liver cell line.