The effect of EDTA on gamma-irradiated female albino mice (Mus musculus)

• Main Author: Llorin, Ryan M.
• Format: text
• Language: English
• Published: Animo Repository 1994
• Subjects: Mice; Effect of radiation on; Ethylenediaminetetraacetic acid; Blood > Analysis and chemistry; EDTA
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/1193
• Institution: De La Salle University

Four groups of 3 female albino mice each were subjected to the following treatments: control group, treatment 1 with EDTA and without gamma rays, treatment 2 without EDTA and with gamma rays and treatment 3 with both EDTA and gamma rays. Two trials were performed for each treatment. EDTA was injected intraperitoneally 25 minutes prior to exposure to 550 rads of gamma radiation. The RBC, WBC and platelet counts and body and spleen weights of the mice were measured. The parameters were counted on the 2nd, 5th and 11th day after the administration of EDTA and gamma radiation. Gamma radiation induced biological damage as manifested in the low blood counts, spleen weights and body weights. EDTA, as a radioprotector, did not prevent the destruction of the elements of the circulating blood and organs but lessened the extent of biological damage and helped in their recovery. However, EDTA, being a toxic substance, produced its own harmful effects in the circulating blood and spleen weight.