Effects of the medicinal plant makabuhay Tinosphora rumphii Boerl. extract on human lymphocytes, in-vitro

• Main Author: De Guzman, Rolando Lizarondo
• Format: text
• Language: English
• Published: Animo Repository 1995
• Subjects: Medicinal plants; Extract; Plants; Useful; Antibacterial agents; Ornamental climbing plants; Lymphocytes
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/1331
• Institution: De La Salle University

Makabuhay Tinosphora rumphii Boerl extracts were derived through decoction methods and thoroughly filtered. The lymphocytes were isolated from the blood through centrifugation methods. The isolated lymphocytes were cultured and treated with mitogen and different concentrations of the extract at 0.28 g/ml and 0.07 g/ml. After 72 hours of incubation, the lymphocyte culture was harvested and counted in terms of live, dead and percent cell viability. The experimental group exhibited a mean cell count and percent viability lower than the positive and negative controls, thus, suggesting that the makabuhay extract at the said concentrations did not exert a proliferative effect, but rather an inhibitory effect on human lymphocytes in-vitro.