Agarose gel electrophoresis patterns of human sera in various disease states

• Main Author: Espiritu, Giselle V.
• Format: text
• Language: English
• Published: Animo Repository 1995
• Subjects: Gel electrophoresis; Separation (Technology); Blood plasma; Blood > Diseases > Diagnosis
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/1335
• Institution: De La Salle University

Serum contains proteins such as albumin and globulins that can be separated into different fractions by agarose gel electrophoresis. The resulting pattern, especially that of samples extracted from patients of a particular disease, is important because it could be used as a gauge to monitor and diagnose that disease.43 samples were obtained and divided as follows: 9 with liver diseases, 4 with heart diseases, 6 with pulmonary diseases, 1 with a neoplastic lung disease, 6 with endocrine diseases, 5 with renal diseases, 1 with tumor, 9 with blood disorders, 1 with disease of the joints and 1 from a drug addict. Data were analyzed by comparing the samples to a normal serum pattern and from results of previous studies.Results showed that proteins were altered as caused by the nature of the disease and were manifested electrophoretically by the difference between each bands of the protein pattern of the sample and the normal serum. The most striking difference from a normal serum sample were seen in multiple myeloma patients while patients with hepatitis B, pulmonary tuberculosis stage 3 with massive hemoptysis, arteriosclerotic heart disease and the drug addict showed no electrophoretic difference from the normal.