Effects of the medicinal plant Blumea balsamifera extract on human lymphocytes, in vitro
The medicinal plant Blumea balsamifera or Sambong used was in tablet form. The extraction was done by pulverization of the tablet and put into boiling for 5 minutes. The lymphocytes were isolated from whole blood through centrifugation methods. The isolated lymphocytes were cultured and treated with...
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| Format: | text |
| Language: | English |
| Published: |
Animo Repository
1995
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| Subjects: | |
| Online Access: | https://animorepository.dlsu.edu.ph/etd_bachelors/1339 |
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| Institution: | De La Salle University |
| Language: | English |
| Summary: | The medicinal plant Blumea balsamifera or Sambong used was in tablet form. The extraction was done by pulverization of the tablet and put into boiling for 5 minutes. The lymphocytes were isolated from whole blood through centrifugation methods. The isolated lymphocytes were cultured and treated with mitogen to serve as positive control, the different concentrations of the extract at 2, 1 and 0.5 to serve as the experimental groups and the untreated lymphocytes to serve as the negative control group. After 72 hours of incubation, the lymphocyte culture was harvested and counted in terms of live and dead cells and percent cell viability. The experimental group exhibited a mean cell count and percent viability lower than the positive and negative controls, thus, suggesting that the sambong extract at the said concentrations did not exert a proliferative but rather an inhibitory effect on human lymphocytes in vitro. |
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