The effect of long-term exogenous insulin and epinephrine administration on hemopoietic cell populations in mice

• Main Author: Gonzales, Rinaldo
• Format: text
• Language: English
• Published: Animo Repository 1996
• Subjects: Insulin; Blood cells; Mice; Adrenalin; Epinephrine
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/1363
• Institution: De La Salle University

To measure the long-term effect of epinephrine and insulin on hemopoietic cell populations on mice, groups of mice were injected with daily subcutaneously administered doses of 0.02-0.04 mg epinephrine and 0.03-0.04 mL of 40U/10 mL Lilly bovine insulin a third group served as control. Long-term epinephrine administration in mice resulted in an increase in total erythrocyte and leukocyte count, an increase in the average percentage level of circulating lymphocytes, as well as substantial weight loss. Insulin-treated mice showed a decreased erythrocyte count, a slight increase in leukocyte levels and an increase in the average percentage level of circulating lymphocytes. Statistical tests using the Student's T-distribution at 0.05 level of significance have shown that long-term administration of epinephrine causes a net decrease in the number of lymphoblasts in the bone marrow of mice and an increase in the number of circulating lymphocytes. Changes in all other blood and bone marrow cell levels (erythrocyte, leukocyte type and blast cells) were not found to be significant for both epinephrine-treated and insulin-treated mice.