Isolation and screening of alkylbenzene-sulfonate-degrading-bacteria in sediments from Laguna de Bay

• Main Author: Sahagun, Rojannah T.
• Format: text
• Language: English
• Published: Animo Repository 1996
• Subjects: Microbial sensitivity tests; Bacterial growth; Water > Microbiology; Water > Pollution; Lake sediments
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/1389
• Institution: De La Salle University

Sediment samples collected from Laguna de Bay were enriched with different concentrations of alkylbenzene sulfonate (ABS), at 2,5, 5.0 and 10.0 mg/L. Bacteria were isolated from the enriched sediment samples and were streaked repeatedly in ABS-overlaid agar plates to ensure purity. Pure isolates were transferred to Mineral Salt Medium (MSM) with their respective ABS concentration and were allowed to incubate for a period of 14 days. The Classic Methylene Blue Method was used to determine the rate of biodegradation. This was done every 14 days until 42 days.Dominant isolates were characterized as Gram negative bacilli, and others had spores. Spore formation could be a means of adaptation of the bacteria to the cultural environment provided during the study.ABS concentrations were computed by applying linear regression to absorbance readings gathered during the periodical confirmatory screening tests. The amount of ABS degraded per day for a period of 28 days was also computed. There was a decline with regards to ABS concentration recorded per incubation period. Bacterial isolates showed degradation of ABS ranging from 0.0142 to 0.01416 mg/L ABS per day.