DNA markers for Capsicum annuum var. Longum

• Main Author: Villanueva, Pauline Michelle S.
• Format: text
• Language: English
• Published: Animo Repository 2005
• Subjects: Genetic markers; Chemistry
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/2040
• Institution: De La Salle University

In this study, crude DNA was isolated from the leaves of Capsicum annuum var. Longum using the CTAB method. The crude DNa'S purity was determined by dividing its average absorbance at גּ260 by its average absorbance at גּ280 and was found to have a value of 0.60. EcoR1 HindIII, and EcoR1/HindIII mix were used for restriction digestion of the DNA before subjecting it to agarose gel electrophoresis and pulsed field get electrophoresis (PEGE) In agarose gel electrophoresis, smeared DNA bands were visualized. Due to smearing and less distinct bands produced in agore gel electrophoresis, the molecular weight of each of the DNA samples was determined using the bands generated from PFGE. IN PFGE, DNA was proven to be fully digested by the enzymes as shown by the longer distance of migration of the digested DNA samples than the distance of migration of the undigested DNA sample. Moreover, it was found out that the undigested DNA sample has a molecular weight of 277.71 kbp while the digested samples all had a similar molecular weight value of 252.37 kbp. For further study, the use of rare cutter restriction enzymes was recommended to minimize smearing of the band. Also, the determination of the molecular markers of the different species of other plants was also recommended to establish a wide variety of genetic information on plants.