The cytotoxic and antimicrobial properties of Angelica keiskei extracts

• Main Author: Reyes, Anna Dominique A.
• Format: text
• Language: English
• Published: Animo Repository 2016
• Subjects: Biology
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/3001
• Institution: De La Salle University

Angelica keiskei is a plant local to the islands of Japan that is more commonly known as Ashitaba. The antimicrobial and cytotoxic activities of the ethanolic extract of combined leaf and stem were determined in the study using the agar disc-diffusion assay and the Presto Blue resazurin cell viability assay, respectively. Angelica keiskei did not inhibit the growth of methicillin resistant Staphylococcus aureus (MRSA), S. aureus ATCC 25923, Escherichia coli ATCC 25922, metallo-β lactamases-producing Pseudomonas aeruginosa, P. aeruginosa ATCC 22853 and extended-spectrum beta-lactamase - producing Klebsiella pneumoniae. On the other hand, Ashitaba demonstrated cytotoxic activities for human small lung cancer cells H69PR (x̅ IC50 21.6 μg/ml) and human colorectal adenocarcinoma HT-29 cells (x̅ IC50 35.5 μg/ml), but was not cytotoxic for the normal human dermal fibroblast HDFn. Using the paired student t-test, the extract was found to have the same potency as the positive control drug, Zeocin (x̅ IC50 40.9 μg/ml), for H69PR cells but was less potent for the HT-29 cells (Zeocin x̅ IC50 27.5 μg/ml).