Conjugative co-transferability of multiple drug resistance and enterotoxigenicity in clinical isolates of Escherichia coli

• Main Authors: Aure, Shaun Frederick M., Mabutas, Ma. Yasmin B.
• Format: text
• Language: English
• Published: Animo Repository 1996
• Subjects: Escherichia coli > Microbiology; Drug resistance in microorganisms; Microorganisms—Effect of antibiotics on; Antibiotics—Physiological effect; Biology
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/4146
• Institution: De La Salle University

Twelve (12) Escherichia coli isolates from San Lazaro Hospital and Escherichia coli SF800 were analyzed for their antibiograms. Among the drugs tested were nalidixic acid, streptomycin, tetracycline, chloramphenicol, sulfamethoxazole trimethoprim, ampicillin and kanamycin. No isolate was found to have resistance pattern to kanamycin. Escherichia coli isolates from San Lazaro Hospital were used as donors for conjugation because of their multiple drug resistance and sensitivity to nalidixic acid. Escherichia coli SF800 which is resistant to nalidixic acid and sensitive to the other antibiotics was used as recipient.In the Ligated Ileal Loop Assay, no fluid accumulation was observed in the loop containing Escherichia coli SF800, implying that enterotoxin was not produced. The twelve donor isolates produced enterotoxin.In the conjugation experiment, nine (9) out of the 12 donors partially transferred their resistance patterns and all 12 donors transferred their enterotoxigenicity to the recipient Escherichia coli SF800. Nine (9) out of the 12 donor isolates of Escherichia coli were able to co-transfer multiple drug resistance and enterotoxigenicity.