Polymerase chain reaction based detection of 1-aminocyclopropane-1-carboxylate deaminase gene in selected strains of pink pigmented facultative methylotrophic bacteria

• Main Authors: Tan, Jayne Sari D., Grecia, Santino P.
• Format: text
• Language: English
• Published: Animo Repository 2009
• Subjects: Deamination; Methylobacterium; Bacteria; Biology
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/5311
• Institution: De La Salle University

Fifty-seven (57) PPFM bacterial isolates deposited at the DLSU Microbiology Laboratory were screened for the presence of ACC deaminase gene. Primers were designed using reported gene sequences from several plant growth promoting bacteria in the National Center for Biotechnological Information (NCBI). Four out of the fifty seven bacterial isolates yielded amplicons corresponding to the expected size of the target gene fragments. The root elongation assay revealed that there was a significant difference in the length of the roots of Vigna radiata (mungbean) grown with bacterial isolates Methylobacterium sp. 14a, Methylobacterium sp. C18, and Methylobacterium sp. C20 compared to the blank and negative control groups. Methylobacterium oryzae CBMB20, the strain obtained from the Korean Agriculture Culture Collection, although did not yield amplicon corresponding to the ACC deaminase gene using the designed primer, it however, showed a significant increase in mungbean root length.