A diffusion study of quercetin and trans-resveratrol using bacterial cellulose as matrix

• Main Authors: Cabusas, Migeul Jemuel O., Kim, Eunjin
• Format: text
• Language: English
• Published: Animo Repository 2016
• Subjects: Cellulose > Chemistry; Quercetin; Chemistry
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/14968
• Institution: De La Salle University

Bacterial cellulose (BC) is characterized by its high purity, strength, moldability and water holding capacity and thus, industries have used BC in several biotechnology and medical applications. In this experiment, the ability of BC to diffuse two polyphenols, queretin and trans-resveratol (tRSV), was investigated. Three set-ups were prepared to monitor the diffusion of the polyphenols, which are polyphenol incorporated bacterial cellulose (BC-tRSV or BC-Quercetin with polyphenol solution in the upper chamber) (Set-up 1), pure BC with polyphenols in the upper chamber (Set-up 2) and polyphenols incorporated in BC (Set-up 3). The results obtained from the experiment were opposite. No significant diffusion was observed in the three setups of quercetin while trans-resveratol showed a significant diffusion in all three setups. Quercetin has more hydroxyl groups than trans-resveratrol, which directly contribute to the diffusion with bacterial cellulose as matrix. Quercetin can form more extensive intramolecular hydrogen bonding with microfibrils of BC which cause the quercetin to be trapped in the fibril network of the BC. On the other hand, tRSV has lower degree of hydroxyl groups and therefore interacts less and form lesser hydrogen bonding with the fibrils. Furthermore, the setup 1 of tRSV diffusion experiment indicated that the diffusion through the BC (setup2) and the diffusion from the BC (setup 3) both contribute to the diffusion of tRSV, which was quantified with HPLC. The diffusion of tRSV was further confirmed with LCMS. Thus, it can be concluded that molecules with more hydroxyl groups may form hydrogen bonds with the BC, thus causing the molecules to be trapped in the pore and slowing or preventing the diffusion significantly. Therefore, BC can be utilized as natural, affordable, and effective delivery system.