Evaluation and effectiveness of the anti-inflammatory and analgesic effects of Garcinia mangostana linn. (mangosteen) on Rattus norvegicus (albino rats) using carrageenan-induced paw edema and writhing test

• Main Authors: Aquino, Miguel Carlos A., Baylon, Jose Lorenzo P.
• Format: text
• Language: English
• Published: Animo Repository 2018
• Subjects: Mangosteen; Rats > Philippines; Albinos and albinism
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/8251
• Institution: De La Salle University

Several studies have shown results regarding the different pharmacological properties and activities that are exhibited by the grinded fruit hull of G. mangostana. The aim of this study was to investigate and observe the presence and effectiveness of the anti-inflammatory and analgesic activities of the Mangosteen fruit pericarp and compare it to the standard drug Mefenamic acid. Mangosteen is commonly known for being used as a traditional folk medicine to treat wound infections, abdominal pains, chronic ulcers, and others in southeast asian countries such as the Philippines. This experiment aims to examines the nociceptive response using acetic acid-induced writhing test and the anti-inflammatory activity of G. mangostana using carrageenan-induced paw edema by using R. norvegicus as experimental animal and post-experimental descriptive histological analysis was also performed to further observe the anti-inflammatory activity of G. mangostana. The experimental animals were separated into 4 test groups, Group A: Distilled water, Group B: low dosage (500 mg/kg), Group C: high dosage (1000 mg/kg), and Group D: Mefenamic acid. The lyophilized fruit hull of G. mangostana (500 mg/kg, 1000 mg/kg) was suspended in an aqueous solution and fed to Group B and C at the required dosage orally 30 minutes before the protocols were done. Although small inhibitory activity is observed this did not significantly inhibit the writings induced by acetic acid. Additionally, the anti-inflammatory activity of G. mangostana extract (500 mg/kg, 1000 mg/kg) was also observed with no statistical significance. Histological analysis shows that leukocyte aggregations were less prominent in rats treated fed with the mangosteen aqueous solution however, this data can still be improved upon. Even though the results shows that G. mangostana exhibits a small amount of analgesic and anti-inflammatory actions, statistical analysis proves that there has no significant statistical difference from the untreated group.