Characterization of selected facultive methylotrophic (PPFM) bacteria deposited at the DLSU-Manila microbiology laboratory

• Main Authors: Anyog, Marielle Tiffany E., Bautista, Grace Celine D., Illustre, Aprille Kirsten P., Mativo, Charmaine A.
• Format: text
• Language: English
• Published: Animo Repository 2008
• Subjects: Methylotrophic microorganisms; Microbiology > Laboratory manuals; Bacterial diseases of plants
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/8347
• Institution: De La Salle University

A total of 32 Pink Pigmented Facultative Methylotrophic (PPFM) bacterial isolates identified as Methylobacterium spp. deposited at the DLSU Microbiology Laboratory were subjected to Fatty Acid Methyl Ester (FAME) Analysis and genotypic characterization. Genotypic characterization included 16s rDNA sequence analysis and miaA gene detection. FAME Analysis revealed that the fatty acid profiles of the isolates confirmed their identity to belong to genus Methylobacterium. 16s rRNA gene analysis revealed that the isolates can be clustered into two groups. Isolates from plants mostly comprise of Methylobacterium radiotoleransformed one cluster, while isolates from soil mostly consisted of Methylobacterium populi formed another cluster. Three out of the 32 isolates showed 300-400 bp amplicon after Polymerase Chain Reaction (PCR) suggesting the presence of miaA gene. Isolates positive for miaA gene were subjected to growth assay using corn as test organism. Results however showed that growth rates of corn cultivated with PPFM bacterial isolates were significantly lower compared to those grown in sterile water or sterile culture media.