Determination of secondary metabolites in germinated mungbeans

• Main Authors: Ramirez, Carlexine Giselle M., Tan Jaiphen, Jaime L.
• Format: text
• Language: English
• Published: Animo Repository 2010
• Subjects: Mung bean; Beans; Germination
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/8475
• Institution: De La Salle University

Mungbeans (Vigna radiata) were germinated and sprouts were studied during stages when they are normally harvested for human consumption. The sprouts were collected at different intervals (0, 1, 2, 3 and 4 days after germination), freeze-dried and ground prior to solvent extraction. Methanol extracts were characterized for secondary metabolite production by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Preparative TLC was done on four-day old sprouts (SM4) where a prominent band of an unknown metabolite, not found in raw seeds, was observed. The isolate was analyzed by infrared spectroscopy. Dichloromethane extracts were analyzed by TLC and Gas Chromatography-Mass Spectrometry. Glucosinolate extracts were prepared and analyzed by HPLC. The results of this study showed changes in metabolites at different stages of germination. Many compounds found in the mature weeds were absent in sprouts or were seen to form only in samples that were allowed to germinate for longer periods. Relative quantification of the data revealed dynamic changes in the amounts of certain metabolites in the course of germination. Prolonged germination resulted in visible physical changes in the seed including the formation of roots and leaves and enhanced secondary metabolite production.