Analysis of DNA damage among urban female street sweepers expose to vehicular exhaust

Occupational exposure to vehicular exhaust in Metro Manila, Philippines is a major human health risk concern because of the established DNA damaging potential of some of its components like PAHs. Hence, in this study, peripheral blood leucocytes of 50 urban female street sweepers/exposed subjects an...

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Main Author: Villarino, Annabella Gorospe
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Language:English
Published: Animo Repository 2015
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Online Access:https://animorepository.dlsu.edu.ph/etd_doctoral/1183
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Institution: De La Salle University
Language: English
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spelling oai:animorepository.dlsu.edu.ph:etd_doctoral-21842024-05-18T06:41:36Z Analysis of DNA damage among urban female street sweepers expose to vehicular exhaust Villarino, Annabella Gorospe Occupational exposure to vehicular exhaust in Metro Manila, Philippines is a major human health risk concern because of the established DNA damaging potential of some of its components like PAHs. Hence, in this study, peripheral blood leucocytes of 50 urban female street sweepers/exposed subjects and an equal number of housekeepers and housewives/reference group were analyzed for DNA damage utilizing the Micronucleus (MN) test and the alkaline single cell gel electrophoresis (SCGE) or comet assay as biomarkers. This study also determined the influence of some demographic characteristics like age, length of fuel exhaust exposure, smoking and alcohol/coffee/tea drinking on DNA damage. Possible association of DNA damage and hematological parameters to include RBC count, WBC count, hematocrit, hemoglobin, lymphocytes and segmenters was also made. Results showed that exposure to vehicular exhaust has caused an increase in tail lengths (8.48±3.41 μm versus 19.35±8.79 μm) and tail moments (1.93±1.43 versus 8.02±5.71) of the leucocytes as demonstrated by the comet assay, but such increase was not observed in micronucleation. Differences in the demographic characteristics of the study population were not significant (p > 0.05) but comet assay results of the smokers, alcohol/coffee/tea drinkers with longer length of exposure to fuel exhaust recorded higher DNA damage compared to the smokers (p < 0.05), alcohol/coffee/tea drinkers of the reference group. Hematological parameters were not affected by fuel exhaust exposure (p > 0.05). Results of the current study suggest the possibility that constant exposure to fuel exhaust could lead to a transient increase in the levels of damage in the DNA of leucocytes and that the comet assay was a particularly sensitive technique in detecting such effects. Other studies are needed to better understand the degree of fuel exhaust exposure needed and the mechanism involved so that the DNA damage may be translated into micronucleus formation. 2015-01-01T08:00:00Z text https://animorepository.dlsu.edu.ph/etd_doctoral/1183 Dissertations English Animo Repository DNA damage Street cleaners Women employees Automobiles—Pollution control devices Biology
institution De La Salle University
building De La Salle University Library
continent Asia
country Philippines
Philippines
content_provider De La Salle University Library
collection DLSU Institutional Repository
language English
topic DNA damage
Street cleaners
Women employees
Automobiles—Pollution control devices
Biology
spellingShingle DNA damage
Street cleaners
Women employees
Automobiles—Pollution control devices
Biology
Villarino, Annabella Gorospe
Analysis of DNA damage among urban female street sweepers expose to vehicular exhaust
description Occupational exposure to vehicular exhaust in Metro Manila, Philippines is a major human health risk concern because of the established DNA damaging potential of some of its components like PAHs. Hence, in this study, peripheral blood leucocytes of 50 urban female street sweepers/exposed subjects and an equal number of housekeepers and housewives/reference group were analyzed for DNA damage utilizing the Micronucleus (MN) test and the alkaline single cell gel electrophoresis (SCGE) or comet assay as biomarkers. This study also determined the influence of some demographic characteristics like age, length of fuel exhaust exposure, smoking and alcohol/coffee/tea drinking on DNA damage. Possible association of DNA damage and hematological parameters to include RBC count, WBC count, hematocrit, hemoglobin, lymphocytes and segmenters was also made. Results showed that exposure to vehicular exhaust has caused an increase in tail lengths (8.48±3.41 μm versus 19.35±8.79 μm) and tail moments (1.93±1.43 versus 8.02±5.71) of the leucocytes as demonstrated by the comet assay, but such increase was not observed in micronucleation. Differences in the demographic characteristics of the study population were not significant (p > 0.05) but comet assay results of the smokers, alcohol/coffee/tea drinkers with longer length of exposure to fuel exhaust recorded higher DNA damage compared to the smokers (p < 0.05), alcohol/coffee/tea drinkers of the reference group. Hematological parameters were not affected by fuel exhaust exposure (p > 0.05). Results of the current study suggest the possibility that constant exposure to fuel exhaust could lead to a transient increase in the levels of damage in the DNA of leucocytes and that the comet assay was a particularly sensitive technique in detecting such effects. Other studies are needed to better understand the degree of fuel exhaust exposure needed and the mechanism involved so that the DNA damage may be translated into micronucleus formation.
format text
author Villarino, Annabella Gorospe
author_facet Villarino, Annabella Gorospe
author_sort Villarino, Annabella Gorospe
title Analysis of DNA damage among urban female street sweepers expose to vehicular exhaust
title_short Analysis of DNA damage among urban female street sweepers expose to vehicular exhaust
title_full Analysis of DNA damage among urban female street sweepers expose to vehicular exhaust
title_fullStr Analysis of DNA damage among urban female street sweepers expose to vehicular exhaust
title_full_unstemmed Analysis of DNA damage among urban female street sweepers expose to vehicular exhaust
title_sort analysis of dna damage among urban female street sweepers expose to vehicular exhaust
publisher Animo Repository
publishDate 2015
url https://animorepository.dlsu.edu.ph/etd_doctoral/1183
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