Plasmid - mediated drug resistance among the intestinal microflora of antibiotic - fed pigs
Ampicillin, gentamycin, kanamycin and tetracycline resistant Escherichia coli and Providencia stuartii isolated from pig intestines were tested for in vitro transfer of their resistance plasmids through conjugation with the resident Escherichia coli of the human intestinal tract. All four replicatio...
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| Format: | text |
| Language: | English |
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Animo Repository
1982
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| Online Access: | https://animorepository.dlsu.edu.ph/etd_honors/11 |
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| Institution: | De La Salle University |
| Language: | English |
| Summary: | Ampicillin, gentamycin, kanamycin and tetracycline resistant Escherichia coli and Providencia stuartii isolated from pig intestines were tested for in vitro transfer of their resistance plasmids through conjugation with the resident Escherichia coli of the human intestinal tract. All four replications indicated negative plasmid transfer. Nonconjugative plasmids may be due to the lack of RTF or the chromosomal integration of R determinant. Among other factors, ultraviolet irradiation failed to induce plasmid transfer but resulted in the loss of ampicillin resistance. The curing effect of UV, in addition to drug resistance multiplicity, clearly illustrates the plasmid nature of drug resistance in the donor organisms isolated for this study. |
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