Biological activities of skullcapflavone I from Andrographis paniculata (Burm.f.) nees on immortalized cell lines and its effect on the expression of key repair and pro-apoptotic genes

• Main Author: Martinez, Joemar D.
• Format: text
• Language: English
• Published: Animo Repository 2020
• Subjects: Scutellaria; Cancer cells; Cell lines; Biology
• Online Access: https://animorepository.dlsu.edu.ph/etd_masteral/5981; https://animorepository.dlsu.edu.ph/cgi/viewcontent.cgi?article=13085&context=etd_masteral
• Institution: De La Salle University

The high prevalence rate of cancer cases worldwide and the adverse side effects of the different cancer treatments encourages researches to find novel plant compounds with promising anti-cancer property. In this study, the biological activities of skullcapflavone I (SKI), a pure compound extracted from the roots of Andrographis paniculata, was evaluated on different cancer cell lines: colorectal (HT-29), breast (MCF-7), leukemia (THP-1), and lung (H69PR); and normal cell line, human neonatal dermal fibroblast (HDFn). PrestoBlue® resazurin viability assays showed that SKI was cytoprotective to HDFn cells against H2O2-induced damage (EC50 = 24.61 µg/mL). PrestoBlue® cell viability assays likewise revealed the cytotoxic activity of SKI against the tested cancer cell lines (IC50 = 3.856 to 9.967 µg/mL), but was significantly not cytotoxic to normal HDFn cells (IC50 > 100 µg/mL). Moreover, SKI exhibited significant genotoxicity in HT-29 cells based on the: tail length (TL) = 41.70 ± 2.65; % tail DNA (D) = 37.95 ± 2.07; tail moment (TM) = 17.93 ± 1.83, of the comets using Comet assay. Furthermore, treatment of HT-29 cells with the IC50 of SKI resulted in the significant up-regulation of the proapoptotic cfos and cjun genes and down-regulation of the key repair genes Akt and Bcl-2, which may explain its experimental cytotoxic and genotoxic actions. From these analyses, it can be inferred that SKI is a promising compound for use as a chemo-preventive agent on normal cells, and as chemotherapeutic agent against different cancer cell lines.