Cytotoxic activity of Terminalia microcarpa decne fruit extract on human cancer and normal call lines and the effect of expression of ealy apoptotic cfos and cjun genes and proinflammatory cytokine interleukin-1 β and tumor necrosis factor-a-genes

• Main Author: Zamora, Pamela Angel D.
• Format: text
• Language: English
• Published: Animo Repository 2020
• Subjects: Terminalia; Traditional medicine; Cancer cells; Cell lines; Cytokines; Biology
• Online Access: https://animorepository.dlsu.edu.ph/etd_masteral/5986; https://animorepository.dlsu.edu.ph/context/etd_masteral/article/13086/viewcontent/Zamora_PamelaAngel_11696613_Partial.pdf
• Institution: De La Salle University

Terminalia microcarpa D., locally known as Kalumpit, is widely used in traditional medicine. It has been reported that it contains several biological activities including anti-cancer and anti-inflammatory activities. This study aimed to investigate the phytochemical components of crude dichloromethane (DCM) extract isolated from the fruit pulp using GC-MS analysis and their cytotoxic and anti-inflammatory activities on human cancer and normal cell lines. It also determined the mechanisms of these activities. Crude DCM extract exhibited cytotoxic effect against the MCF-7 breast cancer cells, HeLa cervical cancer cells, H69PR lung cancer cells, and VCaP prostate cancer cells, but not to THP-1 acute monocytic leukemia cells and normal HDFn human dermal fibroblasts (IC50 ≥ 100 µg/mL). Following PrestoBlue® reasurin assay, the crude DCM extract showed IC50 values of 2.385, 3.228, 3.732, 8.373 µg/mL for MCF-7, HeLa, H69PR and VCaP cell lines, respectively. Treatment exposure to crude DCM extract of MCF-7 cells induced upregulation of early apoptotic genes cfos and cjun transcripts. Moreover, downregulation of proinflammatory cytokines IL-1β and TNF-α transcripts was observed in lipopolysaccharide (LPS)-treated phorbol myristate acetate (PMA)- induced macrophage after treatment with crude DCM extract. This study provides evidence that the crude DCm extract from T. microcarpa was cytotoxic to the cancer cell lines but not to the normal cell line tested. This study also provides strong evidence that crude DCM extract has an anti-inflammatory effect against LPS-induced macrophages. Keywords: Terminalia microcarpa D., crude dichloromethane extract, anti-cancer, early apoptotic genes, anti-inflammatory, pro-inflammatory cytokines