HSP70 expression, oxidative stress and antioxidant capacity response to temperature stress of Scylla serrata from areas with varying temperature profiles

• Main Author: Garcia, Maria Victoria Faith V.
• Format: text
• Language: English
• Published: Animo Repository 2018
• Subjects: Scylla serrata—Effect of temperature on; Biology
• Online Access: https://animorepository.dlsu.edu.ph/etd_masteral/6309; https://animorepository.dlsu.edu.ph/cgi/viewcontent.cgi?article=13365&context=etd_masteral
• Institution: De La Salle University

The Philippines is a major source of the mangrove crab Scylla serrata. Temperature is an important factor for mangrove crab growth. Three major mangrove crab production sites in the Philippines were chosen for this study: Cagayan, Sorsogon and Oriental Mindoro. Sites were chosen based on its temperature profile and climate type. This study aims to determine differences in thermal stress response of S. serrata populations from sites with different temperature profiles using gene expression and enzyme activity. Differences were reflected in stress response through enzyme markers but not in HSP70 gene expression. Enzyme markers all showed correlation to thermal stress indicating its usefulness as tools for thermal stress evaluation. Mindoro populations were highly stressed compared to Cagayan and Sorsogon populations. Sorsogon populations exhibited stress but showed ability to remediate through increased antioxidant activity. Cagayan populations had the highest tolerance for thermal stress with low HSP70 expression, oxidative stress markers and antioxidant activity. Overall result show there is significant difference among sites and the combined effect of site and temperature. Results complement the East and West genetic population structure based on the populations’ thermal stress response. Eastern populations had higher tolerance to thermal stress than the Western population. Results indicate that S. serrata are ideal species for aquaculture. Matching of population sources to rearing sites must be done to keep thermal-sensitive populations in areas that have similar temperature range to minimize stress and possibly improve crab growth and development.