Diuretic potential and characterization of some secondary metabolites from Eleusine indica

• Main Author: Camacho, Drexel .H
• Format: text
• Language: English
• Published: Animo Repository 1996
• Subjects: Diuretics; Extracts; Separation (Technology); Plant metabolites; Medicinal plants; Solution (Chemistry); Chemistry; Education
• Online Access: https://animorepository.dlsu.edu.ph/etd_masteral/1722
• Institution: De La Salle University

This study investigates the diuretic potential and secondary metabolite profile of Eleusine indica. The crude alcoholic extract was fractionated several times by sequential extraction and by chromatographic separation. The fractions were tested for toxicological and diuretic activity. The crude alcoholic extract is non-toxic at 1000 ug/mL (brine shrimp assay) and at 2,500 mg/Kg bw (mice). Among the four extracts obtained by liquid-liquid partitioning, the aqueous ethanolic fraction was found to possess the highest diuretic activity. Its diuretic activity is significantly higher than the positive standard furosemide as well as some other medicinal plant extracts previously screened. Analysis of the brine excreted indicated the presence of a loop diuretic and an acidifying diuretic. Three osmotic diuretics were identified: NaCl, KCl, and NH4Cl.A bicyclic lactonic terpene with a nine-membered ring is characterized for the first time in genus Eleusine. A C-26 sterol (24-norcholesta-3-ol) previously isolated from a scallop, is likewise characterized for the first time in a terrestrial plant. The isolation of this sterol may contribute to the biosynthetic investigations on the unique C-26 skeleton. Characterization is also described for a fatty acid derived compound.