Alkaloids and triterpenes from Catharanthus roseus (Linn.) Don

• Main Author: Inte, Vic Marie L.
• Format: text
• Language: English
• Published: Animo Repository 1997
• Subjects: Alkaloids; Terpenes > Spectra; Solution (Chemistry); Plants; Useful; Medicinal plants; Biological assay; Chemistry
• Online Access: https://animorepository.dlsu.edu.ph/etd_masteral/1833
• Institution: De La Salle University

Chloroform extraction of the leaves of Catharanthus roseus (Linn.) Don with lead acetate treatment afforded two alkaloids: perivine I subscript 1 and vindoline I subscript 3 and a triterpene: ursolic acid I subscript 11. The acid-base alkaloidal extraction afforded vindoline I subscript 3 two triterpenes: squalene I subscript 26 and oleanolic acid I subscript 23 and a hydrocarbon tetracosane I subscript 12. The structures of the alkaloids were elucidated using UV, IR, 1D and 2D homonuclear and heteronuclear nmr spectroscopy. Only I subscript 1 was characterized using mass spectrometry. The triterpenes and the hydrocarbon were elucidated using superscript 1 H-NMR. Vindoline I subscript 3 did not inhibit the growth of - Pseudomonas aerugenosa, Saccharomyces cerevisiae, Candida albicans, Escherichia coli, Staphylococcus aureus and Bacillus subtilis but showed a significant antimutagenic activity. The data gathered from the two extraction processes are not conclusive enough to determine which procedure works best. Seasonal and geographical differences affect the production of secondary metabolites.