Development and comparative characterization of hydrogel derived from the renal cortex and renal medulla of the decellularized kidney

• Main Author: Mondragon, John Martin S.
• Format: text
• Language: English
• Published: Animo Repository 2022
• Subjects: Kidney cortex; Kidneys; Colloids; Biology
• Online Access: https://animorepository.dlsu.edu.ph/etdm_bio/18; https://animorepository.dlsu.edu.ph/cgi/viewcontent.cgi?article=1019&context=etdm_bio
• Institution: De La Salle University

The development of tissue replacement, especially for the kidneys, remains a supreme discipline owing to its anatomic and physiological complexity. Each region of the kidney extracellular matrix (ECM) possesses unique structural, biochemical, and functional characteristics. This regional specificity of the kidney ECM can potentially affect the properties of the hydrogel produced from each region. In this study, hydrogels derived from the renal cortex and renal medulla of the decellularized porcine kidney were developed, characterized, and compared. Hydrogel production involved a series of procedures such as decellularization, solubilization, neutralization, and gelation. The hydrogels were evaluated in terms of surface topography, swelling capacity, and spectral analysis. Results showed that the hydrogels derived from the renal cortex and renal medulla show similar physicochemical properties such as an interconnected porous hydrogel network, swelling behavior and ratio, and chemical structure. The preliminary results of this work can hopefully aid in the pursuit of tested, biocompatible, and ethical solutions to treat diseased and damaged kidneys.