Serological studies on Philippine naegleria isolates

• Main Authors: Orozco, Glorina Divina P., Natividad, Filipinas F., Enriquez, Gloria L.
• Format: text
• Published: Animo Repository 1991
• Subjects: Naegleria—Philippines; Veterinary serology; Biochemistry, Biophysics, and Structural Biology
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/9126
• Institution: De La Salle University

Mice immunized intravenously (IV) and intraperitoneally (IP) with NSRI and RITM Naegleria isolates produced agglutination titers of 1:256 and 1:643 respectively for both homologous and heterologous antisera. Intraperitoneal immunization with adjuvant produced the same titer as in IV route while no agglutinins were detected via the intranasal route of immunization. Slight difference in the degrees of agglutination between homologous and heterologous reactions suggest that the 2 amoebaisolates are closely related and possibly belong to the same species. Ouchterlony gel diffusion test using RITM and NSRI crude membrane antigens (CMA) produced 4 and 3 homologous precipitin lines respectively. Heterologous reaction produced 3 and 2 precipitin lines for RITM and NSRI CMA. Homologous and heterologous reaction using the soluble antigen (SA) of both isolates produced only one precipitin line suggesting that there are more antigenic determinants in the membrane than in the soluble substances in the cell.