Serological studies on Philippine naegleria isolates

Mice immunized intravenously (IV) and intraperitoneally (IP) with NSRI and RITM Naegleria isolates produced agglutination titers of 1:256 and 1:643 respectively for both homologous and heterologous antisera. Intraperitoneal immunization with adjuvant produced the same titer as in IV route while no a...

Full description

Saved in:
Bibliographic Details
Main Authors: Orozco, Glorina Divina P., Natividad, Filipinas F., Enriquez, Gloria L.
Format: text
Published: Animo Repository 1991
Subjects:
Online Access:https://animorepository.dlsu.edu.ph/faculty_research/9126
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: De La Salle University
id oai:animorepository.dlsu.edu.ph:faculty_research-10332
record_format eprints
spelling oai:animorepository.dlsu.edu.ph:faculty_research-103322023-04-22T01:21:57Z Serological studies on Philippine naegleria isolates Orozco, Glorina Divina P. Natividad, Filipinas F. Enriquez, Gloria L. Mice immunized intravenously (IV) and intraperitoneally (IP) with NSRI and RITM Naegleria isolates produced agglutination titers of 1:256 and 1:643 respectively for both homologous and heterologous antisera. Intraperitoneal immunization with adjuvant produced the same titer as in IV route while no agglutinins were detected via the intranasal route of immunization. Slight difference in the degrees of agglutination between homologous and heterologous reactions suggest that the 2 amoebaisolates are closely related and possibly belong to the same species. Ouchterlony gel diffusion test using RITM and NSRI crude membrane antigens (CMA) produced 4 and 3 homologous precipitin lines respectively. Heterologous reaction produced 3 and 2 precipitin lines for RITM and NSRI CMA. Homologous and heterologous reaction using the soluble antigen (SA) of both isolates produced only one precipitin line suggesting that there are more antigenic determinants in the membrane than in the soluble substances in the cell. 1991-01-01T08:00:00Z text https://animorepository.dlsu.edu.ph/faculty_research/9126 Faculty Research Work Animo Repository Naegleria—Philippines Veterinary serology Biochemistry, Biophysics, and Structural Biology
institution De La Salle University
building De La Salle University Library
continent Asia
country Philippines
Philippines
content_provider De La Salle University Library
collection DLSU Institutional Repository
topic Naegleria—Philippines
Veterinary serology
Biochemistry, Biophysics, and Structural Biology
spellingShingle Naegleria—Philippines
Veterinary serology
Biochemistry, Biophysics, and Structural Biology
Orozco, Glorina Divina P.
Natividad, Filipinas F.
Enriquez, Gloria L.
Serological studies on Philippine naegleria isolates
description Mice immunized intravenously (IV) and intraperitoneally (IP) with NSRI and RITM Naegleria isolates produced agglutination titers of 1:256 and 1:643 respectively for both homologous and heterologous antisera. Intraperitoneal immunization with adjuvant produced the same titer as in IV route while no agglutinins were detected via the intranasal route of immunization. Slight difference in the degrees of agglutination between homologous and heterologous reactions suggest that the 2 amoebaisolates are closely related and possibly belong to the same species. Ouchterlony gel diffusion test using RITM and NSRI crude membrane antigens (CMA) produced 4 and 3 homologous precipitin lines respectively. Heterologous reaction produced 3 and 2 precipitin lines for RITM and NSRI CMA. Homologous and heterologous reaction using the soluble antigen (SA) of both isolates produced only one precipitin line suggesting that there are more antigenic determinants in the membrane than in the soluble substances in the cell.
format text
author Orozco, Glorina Divina P.
Natividad, Filipinas F.
Enriquez, Gloria L.
author_facet Orozco, Glorina Divina P.
Natividad, Filipinas F.
Enriquez, Gloria L.
author_sort Orozco, Glorina Divina P.
title Serological studies on Philippine naegleria isolates
title_short Serological studies on Philippine naegleria isolates
title_full Serological studies on Philippine naegleria isolates
title_fullStr Serological studies on Philippine naegleria isolates
title_full_unstemmed Serological studies on Philippine naegleria isolates
title_sort serological studies on philippine naegleria isolates
publisher Animo Repository
publishDate 1991
url https://animorepository.dlsu.edu.ph/faculty_research/9126
_version_ 1767196971018223616