Effects of crude extracts of various pigments isolated from Spirulina platensis and Chlorella vulgaris on the angiogenesis of chick embryo Chorioallantoic membrane (CAM)

• Main Authors: Biscocho, Haydee Evangeline H., Ching, Johnny A.
• Format: text
• Published: Animo Repository 2016
• Subjects: Spirulina; Chlorella; Chickens—Embryos; Blood-vessels—Growth; Biology
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/11214
• Institution: De La Salle University

This study was conducted to determine the angiogenic effects of different concentrations of phytopigments i.e. phycocyanin from Spirulina platensis and chlorophyll from Chlorella vulgaris on the chorioallantoic membrane (CAM) of developing chick embryo. Findings of study revealed that different concentrations of both crude phytopigments inhibit angiogenesis as shown by the continuous decrease of the vascular density index on the CAM of 10-day old chick embryo. However, Scheffe method showed that only 200 ppm and 300 ppm of crude phycocyanin and all experimental treatments of crude chlorophyll, including the 150 ppm, were able to significantly inhibit angiogenesis. It was also established that there is no significant difference in the angiogenic effects between the two phytopigments.