Optimization of prodigiosin production from Serratia marcescens culture

• Main Authors: Casanova, Jannelle R., Reyes, Lilibeth Salvador
• Format: text
• Published: Animo Repository 2017
• Subjects: Metabolites; Serratia marcescens; Antimalarials
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/11206
• Institution: De La Salle University

Malaria has been one of the leading causes of deaths in developing countries such as the Philippines. In 2015, 100% increase in cases was reported by the Department of Health specifically in rural areas. Prodigiosin, a red pigment produced by several microorganisms such as Streptomyces sp., Vibrio sp. and Serratia sp., is a known an antimalarial agent (Suryavanshi et. al, 2016). In this study, culture conditions for the sponge-associated microorganism Serratia marcescens WW4 was optimized to attain higher prodigiosin production. Culture broths of S. marcescens were monitored for total biomass and prodigiosin yield through optical density measurements at 600 nm at 523 nm, respectively. The effect of culture media, temperature, salinity and incubation time on the prodigiosin production was elucidated, with the optimum conditions employed to obtain the highest yield for prodigiosin.