Diosgenin-induced physicochemical effects on phospholipid membranes

Diosgenin (DGN), a plant sterol found in the family Dioscorea, attracts increasing attention for its various pharmacological activities. With the use of fluorescence and ssNMR spectroscopy, we examined the steroidal structural effect of the sterol on the physicochemical properties of phospholipid bi...

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Main Authors: Ondevilla, Joan Candice V., Mukogawa, Akane, Hanashima, Shinya, Umegawa, Yuichi, Murata, Michio
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Published: Animo Repository 2021
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Online Access:https://animorepository.dlsu.edu.ph/faculty_research/11548
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spelling oai:animorepository.dlsu.edu.ph:faculty_research-112512024-03-25T00:19:09Z Diosgenin-induced physicochemical effects on phospholipid membranes Ondevilla, Joan Candice V. Mukogawa, Akane Hanashima, Shinya Umegawa, Yuichi Murata, Michio Diosgenin (DGN), a plant sterol found in the family Dioscorea, attracts increasing attention for its various pharmacological activities. With the use of fluorescence and ssNMR spectroscopy, we examined the steroidal structural effect of the sterol on the physicochemical properties of phospholipid bilayer membranes consisting of palmitoyloleoylphosphatidylcholine (POPC) and comparing it to a widely studied steroidal sterol, cholesterol (Cho). In diphenylhexatriene (DPH) anisotropy experiment, DGN up to 30 mol% stably bound to the POPC bilayer and induced a similar ordering effect on POPC to Cho. Measurement of membrane hydration using laurdan also revealed a similar tendency for Cho and DGN. These results suggest that DGN below 30 mol% has comparable effects with Cho on basic biomembrane properties. However, in 2H NMR, using a deuterated phospholipid, 10-d2-POPC, revealed slight differences in the ordering effect in POPC bilayer in which DGN somewhat affects the lipid packing. These spectra also show that DGN is oriented parallel to the membrane normal like Cho. 31P NMR of POPC in bilayers revealed that, unlike Cho, DGN altered the interactions of POPC headgroups at higher contents over 30 mol%. 2021-03-01T08:00:00Z text https://animorepository.dlsu.edu.ph/faculty_research/11548 Faculty Research Work Animo Repository Diosgenin—Physiological effect Dioscoreaceae —Analysis Membrane lipids Biochemistry
institution De La Salle University
building De La Salle University Library
continent Asia
country Philippines
Philippines
content_provider De La Salle University Library
collection DLSU Institutional Repository
topic Diosgenin—Physiological effect
Dioscoreaceae —Analysis
Membrane lipids
Biochemistry
spellingShingle Diosgenin—Physiological effect
Dioscoreaceae —Analysis
Membrane lipids
Biochemistry
Ondevilla, Joan Candice V.
Mukogawa, Akane
Hanashima, Shinya
Umegawa, Yuichi
Murata, Michio
Diosgenin-induced physicochemical effects on phospholipid membranes
description Diosgenin (DGN), a plant sterol found in the family Dioscorea, attracts increasing attention for its various pharmacological activities. With the use of fluorescence and ssNMR spectroscopy, we examined the steroidal structural effect of the sterol on the physicochemical properties of phospholipid bilayer membranes consisting of palmitoyloleoylphosphatidylcholine (POPC) and comparing it to a widely studied steroidal sterol, cholesterol (Cho). In diphenylhexatriene (DPH) anisotropy experiment, DGN up to 30 mol% stably bound to the POPC bilayer and induced a similar ordering effect on POPC to Cho. Measurement of membrane hydration using laurdan also revealed a similar tendency for Cho and DGN. These results suggest that DGN below 30 mol% has comparable effects with Cho on basic biomembrane properties. However, in 2H NMR, using a deuterated phospholipid, 10-d2-POPC, revealed slight differences in the ordering effect in POPC bilayer in which DGN somewhat affects the lipid packing. These spectra also show that DGN is oriented parallel to the membrane normal like Cho. 31P NMR of POPC in bilayers revealed that, unlike Cho, DGN altered the interactions of POPC headgroups at higher contents over 30 mol%.
format text
author Ondevilla, Joan Candice V.
Mukogawa, Akane
Hanashima, Shinya
Umegawa, Yuichi
Murata, Michio
author_facet Ondevilla, Joan Candice V.
Mukogawa, Akane
Hanashima, Shinya
Umegawa, Yuichi
Murata, Michio
author_sort Ondevilla, Joan Candice V.
title Diosgenin-induced physicochemical effects on phospholipid membranes
title_short Diosgenin-induced physicochemical effects on phospholipid membranes
title_full Diosgenin-induced physicochemical effects on phospholipid membranes
title_fullStr Diosgenin-induced physicochemical effects on phospholipid membranes
title_full_unstemmed Diosgenin-induced physicochemical effects on phospholipid membranes
title_sort diosgenin-induced physicochemical effects on phospholipid membranes
publisher Animo Repository
publishDate 2021
url https://animorepository.dlsu.edu.ph/faculty_research/11548
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