Evaluation of the potential anti-viral activity of microRNAs in rainbow trout

• Main Authors: Bela-ong, Dennis B., Schyth, Brian Dall, Lorenzen, Niels
• Format: text
• Published: Animo Repository 2013
• Subjects: MicroRNA; Antiviral agents; Host-parasite relationships; Osteichthyes—Virus diseases; Biology; Biotechnology
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/11553
• Institution: De La Salle University

Background: Microribonucleic acids (miRNAs) are small (18-22 nucleotides) endogenous RNAs that potently mediate post-transcriptional silencing of a wide range of genes. They are emerging as critical regulators of cellular processes and some miRNAs have been demonstrated to possess direct antiviral effects. We have previously observed and validated that the fish-specific miRNAs, miR-462 and miR- 731, were among the most highly expressed miRNAs in rainbow trout liver following Viral hemorrhagic septicemia virus (VHSV) infection. These miRNAs were also upregulated in the liver and muscle (vaccination site) of fish vaccinated with a DNA vaccine encoding the glycoprotein gene of VHSV. Recent studies further suggest that the expression of these miRNAs is induced by type I interferons (IFN). Here, we analyzed if miR-462 and miR-731 have antiviral effect contributing to the potent antiviral activity of type I IFNs.