Antibiograms of pink pigmented facultative methylotrophic bacterial isolates from various sources

• Main Authors: Pasamba, Edmundo M., Demigillo, Robert M., Jr., Lee, Anthony C.
• Format: text
• Published: Animo Repository 2007
• Subjects: Antibiosis; Methylobacterium
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/11124
• Institution: De La Salle University

The antibiograms of 120 pink pigmented facultative methylotrophic (PPFM) bacterial isolates deposited at the De La Salle University-Manila microbiology laboratory were de­termined. The isolates were obtained from various sources specifically from the air, plants, polluted water and leaf litter. The protocol as prescribed by the Clinical and Laboratory Standards Institute (CLSI 2006) for Enterobacteriaceae and Pseudomonadaceae were adapted. Due to the slow growth of PPFM bacteria as well as sparse growth in Mueller-Hinton agar, modifications on the protocol were performed. Changes in the procedure included extension of the incubation period to 3-5 d, incubation temperature of 30°C as well as the use of glycerol peptone agar and tryptic soy agar as culture media for antimicrobial susceptibility testing. Results revealed that the PPFM bacterial isolates were susceptible to aminoglycosides, tetracycline, cefoperozone, and piperacillin but were resistant to ampicillin, amoxicillin-clavulanic acid. The antibiograms of the isolates were generally similar with published results but with some disparity in the two test media used. Discordant carabapenem susceptibility was exhibited by majority of the isolates. They were found to be susceptible to imipenem while resistant to meropenem.